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Gene Expression Data
Assay Details
Assay
Reference: J:189955 Star EN, et al., Regulation of retinal interneuron subtype identity by the Iroquois homeobox gene Irx6. Development. 2012 Dec;139(24):4644-55
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6450178
Gene symbol: Tacr3
Gene name: tachykinin receptor 3
Antibody: Anti-Nk3r
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two anti-Tacr3 antibodies were described in the methods Table S1. Either of the following could have been used: MGI:6450079 or MGI:6450081.
Results
Specimen 3A: postnatal month 2-3; Irx6tm1Chow/Irx6+ (more )
Note: Double labeled: red - Tacr3; green - Irx6 (assay MGI:6450171).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 3C , 3A Tacr3 labels type 1 and 2 bipolar cells. Tacr3 co-labeled with Irx6:b-gal positive cells in a subset of bipolar cells.

Specimen 5E: postnatal month 2-3; Irx6tm1Chow/Irx6+ (more )
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 5E Expression was detected in type 1 and 2 bipolar cells.

Specimen 5F: postnatal month 2-3; Irx6tm1Chow/Irx6tm1Chow (more )
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 5F Expression in type 1 and 2 bipolar cells was still present in the mutant retina.

Specimen 9B: postnatal month 2-3; Irx6tm1Chow/Irx6+, Vsx1tm1Mci/Vsx1+ (more )
Note: Double labeled: green - Tacr3; red - Hcn4 (assay MGI:6450186).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 9B , 9C Expression was detected in type 1 and 2 bipolar cells, but not in Hcn4-positive type 3a cell soma.
TS28: retina inner plexiform layer Present Regionally restricted 9B' , 9C' Expression in axon terminals did not overlap between Tacr3 and Hcn4.

Specimen 9E: postnatal month 2-3; Irx6tm1Chow/Irx6tm1Chow, Vsx1tm1Mci/Vsx1+ (more )
Note: Double labeled: green - Tacr3; red - Hcn4 (assay MGI:6450186).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 9E , 9F Ectopic Tacr3 expression was observed in Hcn4-immunolabeled cell somas of putative type 3a cells. Expression in type 1 and 2 bipolar cells was not co-labeled with Hcn4. There was an increased labeling of Tacr3 in sublamina 2.
TS28: retina inner plexiform layer Present Regionally restricted 9E' , 9F' Ectopic Tacr3 expression was observed in Hcn4-immunolabeled putative type 3a axon terminals.

Specimen 9H: postnatal month 2-3; Irx6tm1Chow/Irx6tm1Chow, Vsx1tm1Mci/Vsx1tm1Mci (more )
Note: Double labeled: green - Tacr3; red - Hcn4 (assay MGI:6450186).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 9I , 9H In the double mutant, all of the Hcn4-positive bipolar cells co-immunolabeled with Tacr3. There was a decrease of Tacr3-only immunolabeled cells.
TS28: retina inner plexiform layer Present Regionally restricted 9H' , 9I' Hcn4 and Tacr3 co-immunolabeling was noted in the inner plexiform layer.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory