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Gene Expression Data
Assay Details
Assay
Reference: J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6435218
Gene symbol: Mlh1
Gene name: mutL homolog 1
Antibody: anti-Mlh1 (11697-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5E-WT-pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Triple labeled: red - Mlh1; green - Sycp3 (assay MGI:6435158); blue - Ss18l1 (assay MGI:6435219)
Structure Level Pattern Image Note
TS28: spermatocyte Present 5E WT Staining is seen in potential crossover sites.
Specimen 5E-mut-pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Triple labeled: red - Mlh1; green - Sycp3 (assay MGI:6435158); blue - Ss18l1 (assay MGI:6435219)
Structure Level Pattern Image Note
TS28: spermatocyte Present 5E mut Staining is seen in potential crossover sites; the number of these foci in the mutant is increased compared to the number in wild type.
Specimen 8F-WT: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Mlh1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS25: oocyte Present 8F WT Staining is seen in foci.
Specimen 8F-mut: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - Mlh1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS25: oocyte Present 8F mut Staining is seen in foci. The number of foci is increased in the mutant relative to wild type. This is quantified in Fig. 8G.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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