Sycp1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6435217
Gene symbol:	Sycp1
Gene name:	synaptonemal complex protein 1

Antibody:	anti-Sycp1 (ab15087)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

5C WT ©

Specimen 5C-WT-diplotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C WT

5C mut ©

Specimen 5C-mut-diplotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C mut	An increase in precocious separation of homologous chromosomes is observed in the mutants.

Specimen 8H-WT-leptotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Leptotene

Specimen 8H-WT-zygotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Zygotene

Specimen 8H-WT-pachytene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Pachytene

Specimen 8H-WT-diplotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Diplotene

Specimen 8H-mut-leptotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Leptotene

Specimen 8H-mut-zygotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Zygotene

Specimen 8H-mut-pachytene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Pachytene

Specimen 8H-mut-diplotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Sycp1; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Diplotene	Precocious separation of homologous chromosomes was observed in the mutant. This is quantified in Fig. 8I.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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