Gene Expression Data
Assay Details
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Assay
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Reference:
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J:285380
Goldstein JM, et al., Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Sci Rep. 2019 Dec 9;9(1):18613
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6434027
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Gene symbol:
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Gdf11
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Gene name:
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growth differentiation factor 11
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Reporter:
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GFP
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Probe:
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GFP probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2L left:
embryonic day 10.5; Gdf11em1Awa/Gdf11 +
(more  )
Specimen
2L left:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gdf11em1Awa/Gdf11+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forebrain |
Present |
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2L Gfp left
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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| TS17: limb bud |
Present |
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2L Gfp left
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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| TS17: somite |
Present |
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2L Gfp left
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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| TS17: tail |
Present |
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2L Gfp left
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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Specimen
2L center:
embryonic day 10.5; Gdf11em1Awa/Gdf11 +
(more )
Specimen
2L center:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gdf11em1Awa/Gdf11+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
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2L Gfp center
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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| TS17: somite |
Present |
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2L Gfp center
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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| TS17: tail |
Present |
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2L Gfp center
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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Specimen
2L right:
embryonic day 10.5; Gdf11em1Awa/Gdf11 +
(more )
Specimen
2L right:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gdf11em1Awa/Gdf11+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
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2L Gfp right
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The expression pattern of the Gfp probe was similar to Gdf11 in the knockin line #1B heterozygous embryos. Gfp expression recapitulates Gdf11 expression.
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Analysis Tools