Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:285380
Goldstein JM, et al., Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Sci Rep. 2019 Dec 9;9(1):18613
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6434020
|
Gene symbol:
|
Gdf11
|
Gene name:
|
growth differentiation factor 11
|
Probe:
|
Gdf11 probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
2K left:
embryonic day 10.5
(more )
Specimen
2K left:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2K center:
embryonic day 10.5
(more )
Specimen
2K center:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2K right:
embryonic day 10.5
(more )
Specimen
2K right:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2L left:
embryonic day 10.5; Gdf11em1Awa/Gdf11 +
(more )
Specimen
2L left:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Gdf11em1Awa/Gdf11+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forebrain |
Present |
|
2L Gdf11 left
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
TS17: limb bud |
Present |
|
2L Gdf11 left
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
TS17: somite |
Present |
|
2L Gdf11 left
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
TS17: tail |
Present |
|
2L Gdf11 left
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
Specimen
2L center:
embryonic day 10.5; Gdf11em1Awa/Gdf11 +
(more )
Specimen
2L center:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Gdf11em1Awa/Gdf11+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
|
2L Gdf11 center
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
TS17: somite |
Present |
|
2L Gdf11 center
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
TS17: tail |
Present |
|
2L Gdf11 center
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
Specimen
2L right:
embryonic day 10.5; Gdf11em1Awa/Gdf11 +
(more )
Specimen
2L right:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Gdf11em1Awa/Gdf11+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
|
2L Gdf11 right
|
Expression in the knockin line #1B heterozygous embryo was consistent with the normal expression.
|
|