Antibody:	anti-Sycp1 (A12139)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A' , 4A	In zygotene spermatocytes, staining is continuous in the synapsed part of the synaptonemal complex.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4B' , 4B	In pachytene spermatocytes, staining is continuous in the synapsed part of the synaptonemal complex.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C' , 4C	In diplotene spermatocytes, staining is continuous in the synapsed part of the synaptonemal complex.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D' , 4D , 4G' , 4G	In mutant zygotene spermatocytes, as in wild type, staining is continuous in the synapsed part of the synaptonemal complex.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4H' , 4H , 4E , 4E'	In mutant mid pachytene-like spermatocytes, the synaptonemal complexes are desynapsing prematurely.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4F' , 4I , 4I' , 4F	In mutant late pachytene-like spermatocytes, the synaptonemal complex is completely desynapsed.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4V , 4W	In mutant zygotene spermatocytes, synaptonemal complexes are forming but some instances of synapses with several partners are observed.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4X , 4Y	In mutant pachytene-like spermatocytes, synaptonemal complexes have formed but instances of synapses with several partners are observed.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4aa	Staining is present in zygotene spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4ab	Staining is present in pachytene spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Ambiguous		S4ac	A diplotene spermatocyte was pictured; observed staining (or lack thereof) was not discussed.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4ad	Staining is present mutant zygotene spermatocytes; it is comparable to wild type.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4ae	Staining is present mutant pachytene-like spermatocytes; they are showing signs of arrest at this stage.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4af	Staining is present in mutant pachytene-like spermatocytes, but the synaptonemal complexes have disassembled and the protein has remained localized on the axial elements.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4ah merge , S4ah green	In pachytene spermatocytes, staining is continuous in the synapsed part of the synaptonemal complex.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4ai green , S4ai merge	In mutant pachytene-like spermatocytes, the synaptonemal complexes are desynapsing prematurely and the axial elements are farther apart.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4bl merge , S4bl green	Staining is seen in pachytene spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4bm merge , S4bm green	Staining is seen in mutant early pachytene-like spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4bn merge , S4bn green	Staining is seen in mutant mid pachytene-like spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4bo green , S4bo merge	Staining is seen in mutant late pachytene-like spermatocytes.

Structure	Level	Pattern	Image	Note
TS23: oocyte	Present		S5a	A variety of meiotic stages are observed, quantified in Fig. S5i. Pictured is a pachytene oocyte.

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		S5b	90% of the oocytes have reached the pachytene stage, quantified in Fig. S5j; all chromosome pairs are synapsed. Pictured is a pachytene oocyte.

Structure	Level	Pattern	Image	Note
TS26: oocyte	Present		S5c	The majority of oocytes are at pachytene stage, quantified in Fig. S5k; chromosome pairs remain fully synapsed. Pictured is a pachytene oocyte.

Structure	Level	Pattern	Image	Note
TS27: oocyte	Present		S5d	A variety of meiotic stages are observed, quantified in Fig. S5l. Pictured is a diplotene oocyte.

Structure	Level	Pattern	Image	Note
TS23: oocyte	Present		S5e	A variety of meiotic stages are observed, quantified in Fig. S5i; the distribution is similar to that seen in wild type. Pictured is a pachytene-like oocyte.

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		S5f	In the mutant very few normal pachytene-stage oocytes are observed; instead synaptonemal complex disassembly and signal on unpaired chromosomes is observed. Pictured is a pachytene-like oocyte.

Structure	Level	Pattern	Image	Note
TS26: oocyte	Present		S5g	In the mutant oocytes remain in a pachytene-like state; synaptonemal complex disassembly and signal on unpaired chromosomes is observed. Pictured is a pachytene-like oocyte.

Structure	Level	Pattern	Image	Note
TS27: oocyte	Present		S5h	In the mutant oocytes remain in a pachytene-like state; synaptonemal complex disassembly and signal on unpaired chromosomes is observed. Pictured is a pachytene-like oocyte.