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Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431450
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: anti-SCP3 [Cor 10G11/7] (ab97672)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4A: postnatal (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A , 4A' In zygotene spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4B: postnatal (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4B , 4B' In pachytene spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4C: postnatal (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C' , 4C In diplotene spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4G: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4G , 4G' In mutant zygotene spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4H: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4H , 4H' In mutant mid pachytene-like spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4I: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4I' , 4I In mutant late pachytene-like spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4J: postnatal (more )
Note: Wild type. The authors did not provide any information about the Tex12 antibody, so it is possible they used their rabbit Scyp3 antibody (MGI:5775317) to stain this specimen. Double labeled: red - Sycp3; green - Tex12 (assay MGI:6431466).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4J both , 4J red In pachytene spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4K: postnatal (more )
Note: Wild type. The authors did not provide any information about the Tex12 antibody, so it is possible they used their rabbit Scyp3 antibody (MGI:5775317) to stain this specimen. Double labeled: red - Sycp3; green - Tex12 (assay MGI:6431466).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4K both , 4K red In diplotene spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4N/O: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: The authors did not provide any information about the Tex12 antibody, so it is possible they used their rabbit Scyp3 antibody (MGI:5775317) to stain this specimen. Double labeled: red - Sycp3; green - Tex12 (assay MGI:6431466).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4P , 4N , 4O In mutant pachytene-like spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4S/T: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: The authors did not provide any information about the Tex12 antibody, so it is possible they used their rabbit Scyp3 antibody (MGI:5775317) to stain this specimen. Double labeled: red - Sycp3; green - Tex12 (assay MGI:6431466).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4T , 4S , 4U In mutant pachytene-like spermatocytes, staining is seen in the axial chromosomal elements.
Specimen 4V: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4V , 4W In mutant zygotene spermatocytes, staining is seen in the axial chromosomal elements; synaptonemal complexes are forming but some instances of synapses with several partners are observed.
Specimen 4X: postnatal; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4X , 4Y In mutant pachytene-like spermatocytes, staining is seen in the axial chromosomal elements; synaptonemal complexes have formed but instances of synapses with several partners are observed.
Specimen S4a: postnatal day 35 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4aa Staining is present in zygotene spermatocytes.
Specimen S4b: postnatal day 35 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4ab Staining is present in pachytene spermatocytes.
Specimen S4c: postnatal day 35 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4ac Staining is present in diplotene spermatocytes.
Specimen S4d: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4ad Staining is present mutant zygotene spermatocytes; it is comparable to wild type.
Specimen S4e: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4ae Staining is present mutant pachytene-like spermatocytes; some axial elements are synapsed with several partners.
Specimen S4f: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4af Staining is present in mutant pachytene-like spermatocytes; the synaptonemal complexes have disassembled but staining remains on the axial elements.
Specimen S4h: postnatal day 35 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4ah merge , S4ah red Staining is present on the axial elements of pachytene spermatocytes.
Specimen S4i: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4ai red , S4ai merge In mutant pachytene-like spermatocytes, the synaptonemal complexes are desynapsing prematurely and the axial elements are farther apart.
Specimen S4l: postnatal month 3 (more )
Note: Wild type. The authors used two Sycp3 antibodies; it is possible they used their rabbit Sycp3 antibody (MGI:5775317) to stain this specimen. Triple labeled: red - Sycp3 ; green - Sycp1 (assay MGI:6431397); gray - H2ax (assay MGI:6431474).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bl merge , S4bl red Staining is seen in pachytene spermatocytes.
Specimen S4m: postnatal month 3; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: The authors used two Sycp3 antibodies; it is possible they used their rabbit Sycp3 antibody (MGI:5775317) to stain this specimen. Triple labeled: red - Sycp3 ; green - Sycp1 (assay MGI:6431397); gray - H2ax (assay MGI:6431474).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bm merge , S4bm red Staining is seen in mutant early pachytene-like spermatocytes.
Specimen S4n: postnatal month 3; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: The authors used two Sycp3 antibodies; it is possible they used their rabbit Sycp3 antibody (MGI:5775317) to stain this specimen. Triple labeled: red - Sycp3 ; green - Sycp1 (assay MGI:6431397); gray - H2ax (assay MGI:6431474).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bn red , S4bn merge Staining is seen in mutant mid pachytene-like spermatocytes.
Specimen S4o: postnatal month 3; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: The authors used two Sycp3 antibodies; it is possible they used their rabbit Sycp3 antibody (MGI:5775317) to stain this specimen. Triple labeled: red - Sycp3 ; green - Sycp1 (assay MGI:6431397); gray - H2ax (assay MGI:6431474).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bo red , S4bo merge Staining is seen in mutant late pachytene-like spermatocytes.
Specimen S5a: embryonic day 15.5 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS23: oocyte Present S5a A variety of meiotic stages are observed, quantified in Fig. S5i. Pictured is a pachytene oocyte.
Specimen S5b: embryonic day 17.5 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS25: oocyte Present S5b 90% of the oocytes have reached the pachytene stage, quantified in Fig. S5j. Pictured is a pachytene oocyte.
Specimen S5c: embryonic day 19.5 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS26: oocyte Present S5c The majority of oocytes are at pachytene stage, quantified in Fig. S5k. Pictured is a pachytene oocyte.
Specimen S5d: postnatal day 1 (more )
Note: Wild type. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS27: oocyte Present S5d A variety of meiotic stages are observed, quantified in Fig. S5l. Pictured is a diplotene oocyte.
Specimen S5e: embryonic day 15.5; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS23: oocyte Present S5e A variety of meiotic stages are observed, quantified in Fig. S5i; the distribution is similar to that seen in wild type. Pictured is a pachytene-like oocyte.
Specimen S5f: embryonic day 17.5; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS25: oocyte Present S5f In the mutant very few normal pachytene-stage oocytes are observed; instead synaptonemal complex disassembly is observed. Pictured is a pachytene-like oocyte.
Specimen S5g: embryonic day 19.5; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS26: oocyte Present S5g In the mutant very few normal pachytene-stage oocytes are observed; instead synaptonemal complex disassembly is observed. Pictured is a pachytene-like oocyte.
Specimen S5h: postnatal day 1; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6431397).
Structure Level Pattern Image Note
TS27: oocyte Present S5h In the mutant oocytes remain in a pachytene-like state; synaptonemal complex disassembly is observed. Pictured is a pachytene-like oocyte.
Specimen S6a +/+ leptotene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6431480).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a +/+ leptotene Staining is seen in leptotene spermatocytes, in lateral elements.
Specimen S6a +/+ zygotene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6431480).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a +/+ zygotene Staining is seen in zygotene spermatocytes, in lateral elements.
Specimen S6a +/+ pachytene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6431480).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a +/+ pachytene Staining is seen in pachytene spermatocytes, in lateral elements.
Specimen S6a -/- leptotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6431480).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a -/- leptotene Staining is seen in mutant leptotene spermatocytes, in lateral elements.
Specimen S6a -/- zygotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6431480).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a -/- zygotene Staining is seen in mutant zygotene spermatocytes, in lateral elements.
Specimen S6a -/- pachytene-like: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6431480).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a -/- pachytene-like Staining is seen in mutant pachytene-like spermatocytes, but the lateral elements are short.
Specimen S6b +/+ leptotene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6431482).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6b +/+ leptotene Staining is seen in leptotene spermatocytes, in lateral elements.
Specimen S6b +/+ zygotene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6431482).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6b +/+ zygotene Staining is seen in zygotene spermatocytes, in lateral elements.
Specimen S6b +/+ pachytene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6431482).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6b +/+ pachytene Staining is seen in pachytene spermatocytes, in lateral elements.
Specimen S6b -/- leptotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6431482).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6b -/- leptotene Staining is seen in mutant leptotene spermatocytes, in lateral elements.
Specimen S6b -/- zygotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6431482).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6b -/- zygotene Staining is seen in mutant zygotene spermatocytes, in lateral elements.
Specimen S6b -/- pachytene-like: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6431482).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6b -/- pachytene-like Staining is seen in mutant pachytene-like spermatocytes, but the lateral elements are short.
Specimen S6c +/+ leptotene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Rad51 (assay MGI:6431485).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c +/+ leptotene Staining is seen in leptotene spermatocytes, in lateral elements.
Specimen S6c +/+ zygotene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Rad51 (assay MGI:6431485).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c +/+ zygotene Staining is seen in zygotene spermatocytes, in lateral elements.
Specimen S6c +/+ pachytene: postnatal day 35 (more )
Note: Double labeled: red - Sycp3; green - Rad51 (assay MGI:6431485).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c +/+ pachytene Staining is seen in pachytene spermatocytes, in lateral elements.
Specimen S6c -/- leptotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Rad51 (assay MGI:6431485).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c -/- leptotene Staining is seen in mutant leptotene spermatocytes, in lateral elements.
Specimen S6c -/- zygotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Rad51 (assay MGI:6431485).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c -/- zygotene Staining is seen in mutant zygotene spermatocytes, in lateral elements.
Specimen S6c -/- pachytene-like: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: red - Sycp3; green - Rad51 (assay MGI:6431485).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c -/- pachytene-like Staining is seen in mutant pachytene-like spermatocytes, but the lateral elements are short.
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