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Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431485
Gene symbol: Rad51
Gene name: RAD51 recombinase
Antibody: anti-RAD51
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S6c +/+ leptotene: postnatal day 35 (more )
Note: Double labeled: green - Rad51; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c +/+ leptotene Expression is present in recombination nodules.

Specimen S6c +/+ zygotene: postnatal day 35 (more )
Note: Double labeled: green - Rad51; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c +/+ zygotene Expression is present in recombination nodules.

Specimen S6c +/+ pachytene: postnatal day 35 (more )
Note: Double labeled: green - Rad51; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c +/+ pachytene Expression is present in recombination nodules.

Specimen S6c -/- leptotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Rad51; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c -/- leptotene Expression is present in recombination nodules; the number of foci is similar in mutants and wild type.

Specimen S6c -/- zygotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Rad51; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c -/- zygotene Expression is present in recombination nodules; the number of foci is similar in mutants and wild type.

Specimen S6c -/- pachytene-like: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Rad51; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6c -/- pachytene-like Expression is present in recombination nodules; the number of foci is similar in mutants and wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory