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Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431474
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phosphoH2AX (#05-636; JBW301)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen S4l: postnatal month 3 (more )
Note: Wild type. Triple labeled: gray - H2ax; green - Sycp1 (assay MGI:6431397); red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bl merge , S4bl gray In pachytene spermatocytes, staining is seen on the chromatin of the unsynapsed XY bivalent.
Specimen S4m: postnatal month 3; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Triple labeled: gray - H2ax; green - Sycp1 (assay MGI:6431397); red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bm gray , S4bm merge As in wild type, in mutant early pachytene-like spermatocytes, staining is seen on the chromatin of the unsynapsed XY bivalent.
Specimen S4n: postnatal month 3; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Triple labeled: gray - H2ax; green - Sycp1 (assay MGI:6431397); red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bn merge , S4bn gray As in wild type, in mutant mid pachytene-like spermatocytes, staining is seen on the chromatin of the unsynapsed XY bivalent.
Specimen S4o: postnatal month 3; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Triple labeled: gray - H2ax; green - Sycp1 (assay MGI:6431397); red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S4bo gray , S4bo merge As in wild type, in mutant late pachytene-like spermatocytes, staining is seen on the chromatin of the unsynapsed XY bivalent.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory