Antibody:

anti-cleaved Caspase-3 (8G10; #9665S)

Structure	Level	Pattern	Image	Note
TS20: neocortex	Ambiguous		2E E11 ctrl

Structure	Level	Pattern	Image	Note
TS20: neocortex	Absent		2E E11 mut

Structure	Level	Pattern	Image	Note
TS20: neocortex	Ambiguous		2F E12 ctrl

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present		2F E12 mut	The onset of cell death occurred at E12 in the mutant pallium.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Ambiguous		4D E12 ctrl

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present		4D E12 mut	The majority of H2ax-immunopositive cells in the mutant pallium were pyknotic, and 93.2% were also cleaved-Casp3-positive.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	6D	Immunofluorescence with cleaved Casp3 was used to mark apoptotic cells. TUNEL-positive cells marked cells in late stages of apoptosis. Of the cleaved Casp3+/TUNEL- cells in the E12 mutant pallium, 38% were also EdU+.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	6E	Virtually no cleaved Casp3+ cells were positively stained with Aurkb (2.1%).

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Regionally restricted	S2B' , S2B''' , S2B	The widespread cytoplasmic staining in Pax6+ radial glial cells was co-localized with cleaved-Casp3 staining.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Ambiguous		S2C ctrl

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	S2C mut	In the E12 mutant pallium, the vast majority (93.4%) of the cleaved Casp3+ cells were pyknotic. Of the nonpyknotic Casp3+ cells, 4.44% were Tubb3-immunopositive, which was not significantly higher than the control.