About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:166528 Zacharias AL, et al., Canonical Wnt/beta-catenin signaling is required for maintenance but not activation of Pitx2 expression in neural crest during eye development. Dev Dyn. 2010 Dec;239(12):3215-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6388681
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Antibody: P2R10
Detection system: Biotinylated secondary antibody/[Strept]avidin
Results
Specimen 2B/C: embryonic day 10.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Pitx2; green - Ctnnb1 (assay MGI:6388680).
Structure Level Pattern Image Note
TS17: eye mesenchyme Present Regionally restricted 2C , 2B Authors report comparable expression in Ctnnb1-ncko and control littermates.
TS17: head mesenchyme derived from neural crest Present Regionally restricted 2B , 2C Levels of expression sometimes appear diminished in individual neural crest cells.
Specimen 2D: embryonic day 10.5; Ctnnb1tm2Kem/Ctnnb1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Littermate control; embryo was either fl/fl or fl/+. Double labeled: red - Pitx2; green - Ctnnb1 (assay MGI:6388680).
Structure Level Pattern Image Note
TS17: eye mesenchyme Present Regionally restricted 2D Expression in perioptic mesenchyme.
TS17: head mesenchyme derived from neural crest Present 2D
Specimen 2F/G: embryonic day 11.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Pitx2; green - Ctnnb1 (assay MGI:6388680).
Structure Level Pattern Image Note
TS19: perioptic mesenchyme Present 2G , 2F Authors report mesoderm expression is unaffected compared with control.
TS19: head mesenchyme derived from neural crest Weak 2F , 2G Expression dramatically reduced specifically in neural crest cells compared to control littermates.
Specimen 2H: embryonic day 11.5; Ctnnb1tm2Kem/Ctnnb1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Littermate control; embryo was either fl/fl or fl/+. Double labeled: red - Pitx2; green - Ctnnb1 (assay MGI:6388680).
Structure Level Pattern Image Note
TS19: perioptic mesenchyme Present 2H
TS19: head mesenchyme derived from neural crest Present 2H
Specimen 2J/K: embryonic day 12.5; Ctnnb1tm2Kem/Ctnnb1tm2Kem, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Pitx2; green - Ctnnb1 (assay MGI:6388680).
Structure Level Pattern Image Note
TS20: perioptic mesenchyme Present 2K , 2J Mesoderm expression is unaffected compared with control.
TS20: head mesenchyme derived from neural crest Absent 2J , 2K Expression undetectable specifically in neural crest cells.
Specimen 2L: embryonic day 12.5; Ctnnb1tm2Kem/Ctnnb1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Littermate control; embryo was either fl/fl or fl/+. Double labeled: red - Pitx2; green - Ctnnb1 (assay MGI:6388680).
Structure Level Pattern Image Note
TS20: perioptic mesenchyme Present 2L
TS20: head mesenchyme derived from neural crest Strong Regionally restricted 2L Higher level of expression in neural crest surrounding the eyes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory