Gene Expression Data
Assay Details
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Assay
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Reference:
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J:134435
Anselme I, et al., Defects in brain patterning and head morphogenesis in the mouse mutant Fused toes. Dev Biol. 2007 Apr 1;304(1):208-20
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6382590
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Probe:
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En2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2P:
embryonic day 9.5
(more  )
Note: 22 somites. Double labeled: purple - En2; red - Six3 (assay MGI:6382582).
Specimen
2P:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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22 somites. Double labeled: purple - En2; red - Six3 (assay MGI:6382582).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: midbrain-hindbrain junction |
Strong |
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2P
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High levels of expression at midbrain-hindbrain boundary (MHB).
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| TS15: future hindbrain |
Present |
Graded |
2P
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Expression in broad domain centered on MHB. Decreasing gradients anterior and posterior to MHB. Expression extends more caudally than that of Wnt1.
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| TS15: future midbrain |
Present |
Graded |
2P
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Expression in broad domain centered on MHB. Decreasing gradients anterior and posterior to MHB. Expression extends more caudally than that of Wnt1.
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Analysis Tools
