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Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296592
Gene symbol: Hsd3b
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase cluster
Antibody: anti-3bHSD
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 1C 16.5 dpc CT: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1C 16.5 dpc CT
Specimen 1C 16.5 dpc KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1C 16.5 dpc KO
Specimen 1C 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Present 1C 18.5 dpc CT
Specimen 1C 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Weak 1C 18.5 dpc KO Decreased numbers of expressing cells at 18.5 dpc.
Specimen 1C P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Present 1C P0 CT
Specimen 1C P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Weak 1C P0 KO Decreased numbers of expressing cells.
Specimen 1C P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Present 1C P5 CT
Specimen 1C P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Trace 1C P5 KO Expressing cells almost completely disappeard by P5.
Specimen 5B P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: interstitium of the testis Present 5B P0 CT
TS27: Leydig cell Leydig cell Present 5B P0 CT
Specimen 5B P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: interstitium of the testis Present 5B P0 KO
TS27: Leydig cell Leydig cell Present 5B P0 KO
Specimen 5B P2 CT: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: interstitium of the testis Present 5B P2 CT
TS27: Leydig cell Leydig cell Present 5B P2 CT
Specimen 5B P2 KO: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: interstitium of the testis Ambiguous Regionally restricted 5B P2 KO Authors report effect on Leydig cells in interstitium.
TS27: Leydig cell Leydig cell Ambiguous Regionally restricted 5B P2 KO
Specimen 5B P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: interstitium of the testis Present 5B P5 CT
TS28: Leydig cell Leydig cell Present 5B P5 CT
Specimen 5B P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: interstitium of the testis Ambiguous Regionally restricted 5B P5 KO Authors report almost no Leydig cells.
TS28: Leydig cell Leydig cell Ambiguous Regionally restricted 5B P5 KO
Specimen 7A CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7A CT Expression in somatic cells.
Specimen 7A KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Mki67 (assay MGI:6296593); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7A KO Authors state expression patterns were indistinguishable between mutant control embryos.
Specimen 7C CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - Hsd3b; green - Casp3 (assay MGI:6296597); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7C CT Expression in somatic cells.
Specimen 7C KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - Hsd3b; green - Casp3 (assay MGI:6296597); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7C KO Authors state expression patterns were indistinguishable between mutant control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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