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Gene Expression Data
Assay Details
Assay
Reference: J:163559 Messerschmidt DM, et al., Nanog is required for primitive endoderm formation through a non-cell autonomous mechanism. Dev Biol. 2010 Aug 1;344(1):129-37
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6272734
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4
Detection system: Alexa Fluor 594
Results
Specimen 1H/I: embryonic day 3.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Nanog (assay MGI:6272731); blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 1I , 1H Expression in all cells at early blastocyst stage. Authors state expression was unaffected by the loss of Nanog.
Specimen 1K/L: embryonic day 3.5 (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Nanog (assay MGI:6272731); blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 1K , 1L Expression in all cells at early blastocyst stage.
Specimen 2B/D: embryonic day 4.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Nanog (assay MGI:6272731); blue - DAPI.
Structure Level Pattern Image Note
TS6: trophectoderm Ambiguous 2D , 2B Authors report expression absent in most trophectoderm cells.
TS6: epiblast Present 2A , 2D Authors report expression in the inner cell mass.
Specimen 2F/H: embryonic day 4.5 (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Nanog (assay MGI:6272731); blue - DAPI.
Structure Level Pattern Image Note
TS6: trophectoderm Ambiguous 2F , 2H Authors report expression absent in most trophectoderm cells.
TS6: epiblast Present Regionally restricted 2E , 2H Authors report expression in the inner cell mass, divided in two populations in controls, ovelapping with Nanog.
Specimen 3B/C: embryonic day 3.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Gata4 (assay MGI:6272737); blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present 3B , 3C
Specimen 3E/F: embryonic day 3.5 (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Gata4 (assay MGI:6272737); blue - DAPI.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3F , 3E
Specimen 3H/I: embryonic day 4.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Gata4 (assay MGI:6272737); blue - DAPI.
Structure Level Pattern Image Note
TS6: blastocyst Present 3I , 3H Authors report inner cell mass is disarranged.
Specimen 3K/L: embryonic day 4.5 (more )
Note: Triple labeled: red - Oct4 (Pou5f1); green - Gata4 (assay MGI:6272737); blue - DAPI.
Structure Level Pattern Image Note
TS6: epiblast Present Regionally restricted 3K , 3L Authors report expression in the inner cell mass, divided in two populations in controls, ovelapping with Gata4.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory