Gata4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:163559 Messerschmidt DM, et al., Nanog is required for primitive endoderm formation through a non-cell autonomous mechanism. Dev Biol. 2010 Aug 1;344(1):129-37
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6272737
Gene symbol:	Gata4
Gene name:	GATA binding protein 4

Antibody:	anti-Gata4
Detection system:	Alexa Fluor 488

Results

3A ©

3C ©

Specimen 3A/C: embryonic day 3.5; Nanog^{Gt(D042B07)Wrst}/Nanog^{Gt(D047A12)Wrst} (more

)
Note: Triple labeled: green - Gata4; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		3A , 3C	Authors state mutant embryos were entirely devoid of Gata4-expressing cells.

3D ©

3F ©

Specimen 3D/F: embryonic day 3.5 (more

)
Note: Triple labeled: green - Gata4; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Single cells	3D , 3F	Single expressing cells could be observed in control embryo.

3G ©

3I ©

Specimen 3G/I: embryonic day 4.5; Nanog^{Gt(D042B07)Wrst}/Nanog^{Gt(D047A12)Wrst} (more

)
Note: Triple labeled: green - Gata4; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present	Single cells	3I , 3G	Expression in a few cells in disarranged inner cell mass.

3J ©

3L ©

Specimen 3J/L: embryonic day 4.5 (more

)
Note: Triple labeled: green - Gata4; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		3L , 3J

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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