Nanog^{Gt(D047A12)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Nanog^{Gt(D047A12)Wrst}
• Name: Nanog homeobox; gene trap D047A12, German Gene Trap Consortium
• MGI ID: MGI:3874467
• Synonyms: Nanog^gt2
• Gene: Nanog Location: Chr6:122684448-122691592 bp, + strand Genetic Position: Chr6, 57.8 cM, cytoband F2
• Alliance: Nanog^{Gt(D047A12)Wrst} page

Mutation origin

• Mutant Cell Line: D047A12
• Germline Transmission: Earliest citation of germline transmission: J:163559
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: rFlpROSAbetageo
• Mutation details: The gene trap vector inserted into intron 1. Immunofluorescence confirmed the lack of expression at E3.5. (J:163559)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  3SD047A12	ER899035 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr6:122685237-122685528 bp (+)
  D047A12	DU822657 ; (MGI Seq Detail)	5' RACE	Chr6:122684552-122684891 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  14 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	15 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nanog Mutation: 46 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)