Dnmt1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:135338 Cirio MC, et al., Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints. BMC Dev Biol. 2008;8:9
Assay type:	Western blot
MGI Accession ID:	MGI:6201237
Gene symbol:	Dnmt1
Gene name:	DNA methyltransferase 1

Antibody:	UPT82
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Antibody recognizes only the Dnmt1s proteins.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
+ GV	P21-35	TS28: primary oocyte	Present (a)	Not Specified		Female
+ MII	P21-35	TS28: secondary oocyte	Present	Not Specified		Female
1s/1o MII	P21-35	TS28: secondary oocyte	Present (b)	involves: 129S1/Sv * C57BL/6	Dnmt1^tm1Jamt/Dnmt1^tm1Jamt	Female
delta1o MII	P21-35	TS28: secondary oocyte	Present (c)	involves: 129S1/Sv * FVB/N	Dnmt1^tm1Jrc/Dnmt1^tm1Jrc	Female

Notes:
(a) Present at an approximately 10-fold higher concentration in GV-stage oocytes compared to MII oocytes.
(b) Increased expression compared to the wild-type.
(c) Increased expression (40-fold) compared to the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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