Dnmt1^tm1Jrc
Targeted Allele Detail

Summary

• Symbol: Dnmt1^tm1Jrc
• Name: DNA methyltransferase 1; targeted mutation 1, J Richard Chaillet
• MGI ID: MGI:2387992
• Synonyms: Dnmt1^delta1o
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^tm1Jrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68455
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The oocyte-specific exon 1o and the proximal promoter region were deleted and replaced with a floxed HSV-tk-PGK-neo cassette. The resistance cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Immunohistochemical analysis of ovary sections from homozygous mutant animals confirmed the absence of gene expression in mutant oocytes. (J:68455)

Expression

In Mice Carrying this Mutation:	228 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 108 strains or lines available

References

• Original: J:68455 Howell CY, et al., Genomic imprinting disrupted by a maternal effect mutation in the Dnmt1 gene. Cell. 2001 Mar 23;104(6):829-38
• All: 8 reference(s)