Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:145977
Tetreault N, et al., The LIM homeobox transcription factor Lhx2 is required to specify the retina field and synergistically cooperates with Pax6 for Six6 trans-activation. Dev Biol. 2009 Mar 15;327(2):541-50
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6198684
|
Gene symbol:
|
Rax
|
Gene name:
|
retina and anterior neural fold homeobox
|
Probe:
|
Rx probe3
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1G Rx:
embryonic day 8.25
(more )
Note: 2-somite stage
Specimen
1G Rx:
(close )
Genetic Background:
|
either: 129 or CD-1
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: eye neural ectoderm |
Present |
Regionally restricted |
1G Rx
|
Expression demarcates specific region of anterior neural plate corresponding to presumptive retina field.
|
Specimen
2C -/-:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Lhx2tm1Dra/Lhx2tm1Dra
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: eye neural ectoderm |
Ambiguous |
Regionally restricted |
2C Lhx2-/-
|
Expression absent or highly reduced in anterior neural plate in region corresponding to presumptive retina field.
|
Specimen
2C wt:
embryonic day 8.25
(more )
Specimen
2C wt:
(close )
Genetic Background:
|
either: 129 or CD-1
|
Age:
|
embryonic day 8.25
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: eye neural ectoderm |
Present |
Regionally restricted |
2C wt
|
Expression present in anterior neural plate in region corresponding to presumptive retina field.
|
Specimen
3A -/-:
(close )
Genetic Background:
|
involves: 129S4/SvJae
|
Mutant Allele(s):
|
Lhx2tm1Dra/Lhx2tm1Dra
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A wt:
embryonic day 9.0
(more )
Specimen
3A wt:
(close )
Genetic Background:
|
either: 129 or CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
|