Antibody:	anti-RALDH2
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: abdominal segment of trunk	Ambiguous		2C

Structure	Level	Pattern	Image	Note
TS21: pleural cavity	Present	Regionally restricted	2D	Expression in cells in lateral areas of pleural cavities.

Structure	Level	Pattern	Image	Note
TS17: liver	Moderate	Regionally restricted	6A	Moderate expression in dorsal lobes of liver.
TS17: intraembryonic coelom	Present	Regionally restricted	6A	Expression in cells extending through dorsal and lateral walls of coelomic cavity.

Structure	Level	Pattern	Image	Note
TS17: liver	Moderate	Regionally restricted	6B	Moderate expression in dorsal lobes of liver.
TS17: intraembryonic coelom	Present	Regionally restricted	6B	Expression in cells remain closely associated with dorsal coelomic epithelium, not extending ventrally

Structure	Level	Pattern	Image	Note
TS17: liver	Moderate	Regionally restricted	6C	Moderate expression in dorsal lobes of liver.
TS17: intraembryonic coelom	Present	Regionally restricted	6C	Expression in cells remain closely associated with dorsal coelomic epithelium, not extending ventrally.

Structure	Level	Pattern	Image	Note
TS19: liver	Strong	Regionally restricted	6D	Prominent expression throughout liver coelomic epithelium. Weaker expression in ventral areas.
TS19: intraembryonic coelom	Present	Regionally restricted	6D	Expression in dorsal and dorsolateral coelomic epithelium, as well in mesenchymal cells from coelomic wall, extending toward lateroventral body wall.

Structure	Level	Pattern	Image	Note
TS19: liver	Strong	Regionally restricted	6E	Prominent expression throughout liver coelomic epithelium. Weaker expression in ventral areas of the liver.
TS19: intraembryonic coelom	Present	Regionally restricted	6E	Expression in dorsal and dorsolateral coelomic epithelium, as well in mesenchymal cells from coelomic wall, extending toward lateroventral body wall.

Structure	Level	Pattern	Image	Note
TS19: liver	Weak	Regionally restricted	6F	Weaker expression than in control embryos, mainly restricted to dorsal areas. Very weak or no expression in lateral and ventral areas of the liver.
TS19: intraembryonic coelom	Present	Regionally restricted	6F	Authors state the limit of expression in parietal coelomic epithelium remains in the same place as at former stage, never extending to the lateral body wall.

Structure	Level	Pattern	Image	Note
TS20: liver	Strong	Regionally restricted	6G	Prominent expression throughout liver coelomic epithelium. Weaker expression in ventral areas.
TS20: intraembryonic coelom	Present	Regionally restricted	6G	Expression in dorsal and dorsolateral coelomic epithelium, as well in mesenchymal cells from coelomic wall.

Structure	Level	Pattern	Image	Note
TS20: liver	Weak	Regionally restricted	6H/I	Weaker expression than in control embryos, mainly restricted to dorsal areas. Very weak or no expression in lateral and ventral areas of the liver.
TS20: stomach	Strong		6H/I

Structure	Level	Pattern	Image	Note
TS20: liver	Weak	Regionally restricted	6J	Weaker expression than in control embryos, mainly restricted to dorsal areas. Very weak or no expression in lateral and ventral areas of the liver.
TS20: intestine	Strong		6J

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Regionally restricted	6K	Decreased expression throughout liver coelomic epithelium.
TS21: intraembryonic coelom	Weak	Regionally restricted	6K	Decreased expression in adjacent parietal coelomic epithelium.

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Regionally restricted	6L	Decreased expression throughout liver coelomic epithelium.
TS21: intraembryonic coelom	Weak	Regionally restricted	6L	Decreased expression in adjacent parietal coelomic epithelium.

Structure	Level	Pattern	Image	Note
TS21: liver	Weak	Regionally restricted	6M	Weaker expression than in control embryos, mainly restricted to dorsal areas. Very weak or no expression in lateral and ventral areas of the liver.

Structure	Level	Pattern	Image	Note
TS21: mesonephros	Present		6M insert	Expression in rudimentary mesonephros.
TS21: lung	Present	Regionally restricted	6M insert	Expression in lung surface.