Gene Expression Data
Assay Details
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Assay
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Reference:
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J:28395
Kaneko-Ishino T, et al., Peg1/Mest imprinted gene on chromosome 6 identified by cDNA subtraction hybridization. Nat Genet. 1995 Sep;11(1):52-9
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:6154604
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Gene symbol:
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Mest
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Gene name:
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mesoderm specific transcript
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Probe:
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Mest-pA, Mest-pB
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Assay notes:
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Imprinting verification by RFLP analysis. RT-PCR products were digested with TaqI prior to electrophoresis.
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Results
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Image: 5b
| Mol. Wt. Marker Lane |
| P adult
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TS28: liver
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Present
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Not Applicable
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Present
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Not Applicable
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0.1 µg; total RNA |
M. spretus |
| Male |
| P adult
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TS28: liver
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Not Applicable
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Present
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Not Applicable
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Present
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0.1 µg; total RNA |
C57BL/6 |
| Female |
| E9.5
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TS15: embryo
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Present
(c)
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Absent
(d)
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Present
(c)
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Absent
(d)
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0.1 µg; total RNA |
(C57BL/6 x M. spretus)F1 |
| Not Specified |
| E9.5
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TS15: embryo
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Present
(c)
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Absent
(d)
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Present
(c)
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Absent
(d)
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0.1 µg; total RNA |
(C57BL/6 x M. spretus)F1 |
| Not Specified |
| E9.5
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TS15: embryo
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Present
(c)
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Absent
(d)
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Present
(c)
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Absent
(d)
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0.1 µg; total RNA |
(C57BL/6 x M. spretus)F1 |
| Not Specified |
| Mol. Wt. Marker Lane |
Notes:
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Analysis Tools
