Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		1e E6.5	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was prominent in the epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1e E7.5	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was detected in the anterior half of the gastrula.
TS11: embryo ectoderm	Present	Non-Uniform	1e E7.5	Expression was detected in a mosaic pattern.
TS11: embryo mesoderm	Absent		1e E7.5

Structure	Level	Pattern	Image	Note
TS13: embryo	Weak	Regionally restricted	1e E8.5	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was weak in the head folds.
TS13: neural tube	Weak		1e E8.5

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1f whole mount	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was detected in the anterior half of the gastrula.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1f section	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was detected in the anterior half of the gastrula.
TS11: embryo ectoderm	Present	Non-Uniform	1f section	Expression was detected in a mosaic pattern.
TS11: embryo mesoderm	Absent		1f section

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1g whole mount	Staining was more intense and in broader regions than in the heterozygous littermates.
TS11: extraembryonic component	Present		1g whole mount	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1g section	Expression was detected in all epiblast cells.
TS11: embryo mesoderm	Present		1g section
TS11: primitive endoderm	Absent		1g section

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		S2a 8-cell	Expression was in all blastomeres of 8-cell embryo.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		S2a Morula	Expression was detected in all blastomeres of the morula.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S2a Blastocyst

Structure	Level	Pattern	Image	Note
TS7: epiblast	Strong		S2b E5.5

Structure	Level	Pattern	Image	Note
TS8: epiblast	Strong		S2b E6

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		S2b E6.5	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was prominent in the epiblast.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		S2b E7.5	Expression in the gene trap recapitulated the expression observed by whole mount RNA in situ hybridization where expression was detected in the anterior half of the gastrula.

Structure	Level	Pattern	Image	Note
TS12: embryo	Ambiguous		S2b E8

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	S2b E8.5	Expression was detected in the neuroepithelium of the head fold.
TS13: neural tube	Present		S2b E8.5

Structure	Level	Pattern	Image	Note
TS14: embryo	Ambiguous		S2b E9

Structure	Level	Pattern	Image	Note
TS15: future brain	Weak		S2b E9.5
TS15: urogenital ridge	Weak		S2b E9.5

Structure	Level	Pattern	Image	Note
TS17: embryo	Ambiguous		S2b E10.5

Structure	Level	Pattern	Image	Note
TS7: embryo	Present		S2c E5.5	Staining was more intense and in broader regions than in the heterozygous littermates.
TS7: extraembryonic ectoderm	Present		S2c E5.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		S2c E6.5	Staining was more intense and in broader regions than in the heterozygous littermates.
TS9: extraembryonic ectoderm	Present		S2c E6.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		S2c E7.5	Staining was more intense and in broader regions than in the heterozygous littermates.
TS11: extraembryonic ectoderm	Present		S2c E7.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		S2c E8	Staining was more intense and in broader regions than in the heterozygous littermates.
TS12: extraembryonic ectoderm	Present		S2c E8	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		S2c E8.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		S2c E9.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		S2c E10.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		S2d E7.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		S2d E8.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		S2d E9.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		S2e E8.0	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		S2e E9.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		S2f E6.5	All epiblast cells stained intensely blue.
TS9: extraembryonic ectoderm	Present		S2f E6.5	Many cells in the extraembryonic ectoderm were positive.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		S2f E7.5	All epiblast cells were stained.
TS11: embryo mesoderm	Present		S2f E7.5

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		S2f E8.5	Staining was more intense and in broader regions than in the heterozygous littermates.

Structure	Level	Pattern	Image	Note
TS9: embryo	Strong		S2h	Very intense staining was detected in the epiblast.
TS9: extraembryonic ectoderm	Present		S2h	Lighter staining was observed in the extraembryonic ectoderm.