Tet1^tm1Koh
Targeted Allele Detail

Summary

• Symbol: Tet1^tm1Koh
• Name: tet methylcytosine dioxygenase 1; targeted mutation 1, Kian Peng Koh
• MGI ID: MGI:5911919
• Synonyms: Tet1^lacZ
• Gene: Tet1 Location: Chr10:62640349-62723242 bp, - strand Genetic Position: Chr10, 32.48 cM
• Alliance: Tet1^tm1Koh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243754
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: A target vector was designed to insert an fl-NLS-lacZ-fl-mCherry-FRT-neo-FRT cassette (containing ATG start codon and nuclear localization signal sequence 5 of lacZ reporter gene with 3 BGH polyA, flanked by 2 LoxP sites to allow reporter exchange to a downstream ATG-mCherry-pA cassette by Cre recombination, followed by a FRT-flanked neomycin selection cassette) in-frame at the ATG start codon in exon 2 of the gene Tet1. The targeting construct was electroporated into C57BL/6 mouse embryonic stem (ES) cells and selected in G418. The transgenic KI mouse strain was created by blastocyst injection of one correctly targeted clone. The strain has been bred to Flp-deleter mice to remove the neo cassette, resulting in one remaining FRT site downstream of the fl-NLS-lacZ-fl-mCherry cassette. The final modification is designated as Tet1-fl-lacZ-fl-mCherry. (J:243754)

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tet1 Mutation: 142 strains or lines available

References

• Original: J:243754 Khoueiry R, et al., Lineage-specific functions of TET1 in the postimplantation mouse embryo. Nat Genet. 2017 Jul;49(7):1061-1072
• All: 2 reference(s)