Tet1^{Gt(RRG140)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Tet1^{Gt(RRG140)Byg}
• Name: tet methylcytosine dioxygenase 1; gene trap RRG140, BayGenomics
• MGI ID: MGI:4129024
• Synonyms: Tet1^Gt
• Gene: Tet1 Location: Chr10:62640349-62723242 bp, - strand Genetic Position: Chr10, 32.48 cM
• Alliance: Tet1^{Gt(RRG140)Byg} page

Mutation origin

• Mutant Cell Line: RRG140
• Germline Transmission: Earliest citation of germline transmission: J:194101
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: Three copies of the beta-geo gene trap inserted in tandem between exon 1 and 2. Western blot analysis confirmed the absence of full-length protein in ES cells. A truncated protein is expressed. (J:194101, J:243754)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRG140	CC325906 ; (MGI Seq Detail)	5' RACE	Chr10:62713933-62714389 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  200 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	55 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tet1 Mutation: 142 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

Initially, two copies of the gene trap were detected (J:194101). Sequencent analysis detected three copies of the gene trap (J:243754).

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)