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Gene Expression Data
Assay Details
Assay
Reference: J:239876 Xu Q, et al., Maternal BCAS2 protects genomic integrity in mouse early embryonic development. Development. 2015 Nov 15;142(22):3943-53
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5910436
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-gH2AX (22551)
Detection system: Secondary antibody
Results
Specimen 2A PN2: embryonic day 0.5 (more )
Note: 1-cell zygote 23 h post-hCG. Pronuclear stage 2. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2A PN2 gH2AX , 2A PN2 Merge
Specimen 2A Mid PN3: embryonic day 0.5 (more )
Note: 1-cell zygote 25 h post-hCG. Pronuclear stage mid 3. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2A Mid PN3 Merge , 2A Mid PN3 gH2AX
Specimen 2A PN4: embryonic day 0.5 (more )
Note: 1-cell zygote 28 h post-hCG. Pronuclear stage 4. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2A PN4 gH2AX , 2A PN4 Merge
Specimen 2A late PN5: embryonic day 0.5 (more )
Note: 1-cell zygote 30 h post-hCG. Pronuclear stage late 5. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2A Late PN5 Merge , 2A Late PN5 gH2AX
Specimen 2B PN2: embryonic day 0.5 (more )
Note: 1-cell zygote 23 h post-hCG. Pronuclear stage 2. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2B PN2 gH2AX , 2B PN2 Merge
Specimen 2B Late PN3: embryonic day 0.5 (more )
Note: 1-cell zygote 25 h post-hCG. Pronuclear stage late 3. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2B Late PN3 gH2AX , 2B Late PN3 Merge
Specimen 2B PN4: embryonic day 0.5 (more )
Note: 1-cell zygote 28 h post-hCG. Pronuclear stage 4. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2B PN4 gH2AX , 2B PN4 Merge
Specimen 2B Early PN5: embryonic day 0.5 (more )
Note: 1-cell zygote 30 h post-hCG. Pronuclear stage early 5. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2B Early PN5 Merge , 2B Early PN5 gH2AX
Specimen 2B Late PN5: embryonic day 0.5 (more )
Note: 1-cell zygote 30 h post-hCG. Pronuclear stage late 5. Double labeled: red - H2ax; green - Bcas2 (assay MGI:5910434). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2B Late PN5 Merge , 2B Late PN5 gH2AX
Specimen 4A 33 control: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryos 33 h post-hCG.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 4A gH2AX 33 Hrs Control , 4A 33 Hrs Overview Control
Specimen 4A 37 control: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryos 37 h post-hCG.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 4A gH2AX 37 Hrs Control , 4A 37 Hrs Overview Control
Specimen 4A 48 control: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryos 48 h post-hCG.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 4A gH2AX 48 Hrs Control , 4A 48 Hrs Overview Control
Specimen 4A 33: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryos 33 h post-hCG.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 4A 33 Hrs Overview , 4A gH2AX 33 Hrs
Specimen 4A 37: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryos 37 h post-hCG.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 4A 37 Hrs Overview , 4A gH2AX 37 Hrs
Specimen 4A 48: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryos 48 h post-hCG.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 4A 48 Hrs Overview , 4A gH2AX 48 Hrs
Specimen 5A Control: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryo 48 h post-hCG. Double labeled: red - H2ax; green - Trp53 (assay MGI:5910510).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 5A Control Merge , 5A Control gH2AX
Specimen 5A: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryo 48 h post-hCG. Double labeled: red - H2ax; green - Trp53 (assay MGI:5910510).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 5A Bcas2mNull gH2AX , 5A Bcas2mNull Merge
Specimen 5G Control: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryo 48 h post-hCG. Double labeled: red - H2ax; green - Chek1 (assay MGI:5910517).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 5G Control Merge , 5G Control gH2AX
Specimen 5G: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryo 48 h post-hCG. Double labeled: red - H2ax; green - Chek1 (assay MGI:5910517).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 5G Bcas2mNull Merge , 5G Bcas2mNull gH2AX
Specimen 6A Control PN2: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 14 h post-hCG. Pronuclear stage 2.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 6A Control PN2 , 6A Control Overview PN2
Specimen 6A Control PN3: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 25 h post-hCG. Pronuclear stage 3.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Strong 6A Control PN3 , 6A Control Overview PN3
Specimen 6A Control PN4: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 28 h post-hCG. Pronuclear stage 4.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Strong 6A Control PN4 , 6A Control Overview PN4
Specimen 6A Control PN5: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 28 h post-hCG. Pronuclear stage 5.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 6A Control Overview PN5 , 6A Control PN5
Specimen 6A PN2: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 14 h post-hCG. Pronuclear stage 2.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 6A Bcas2mNull PN2 Overview , 6A Bcas2mNull PN2
Specimen 6A PN3: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 25 h post-hCG. Pronuclear stage 3.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Strong 6A Bcas2mNull PN3 , 6A Bcas2mNull PN3 Overview
Specimen 6A PN4: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 28 h post-hCG. Pronuclear stage 4.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Strong 6A Bcas2mNull PN4 Overview , 6A Bcas2mNull PN4
Specimen 6A PN5: embryonic day 0.5; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 1-cell zygote 28 h post-hCG. Pronuclear stage 5.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Strong 6A Bcas2mNull PN5 , 6A Bcas2mNull PN5 Overview
Specimen S4A 37: embryonic day 1.0 (more )
Note: 2-cell embryo 37 h post-hCG. Double labeled: red - H2ax; green - Trp53 (assay MGI:5910510). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S4A gH2AX 37 , S4A Merge 37
Specimen S4A 48: embryonic day 1.0 (more )
Note: 2-cell embryo 48 h post-hCG. Double labeled: red - H2ax; green - Trp53 (assay MGI:5910510). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S4A Merge 48 , S4A gH2AX 48
Specimen S4B 37: embryonic day 1.0 (more )
Note: 2-cell embryo 37 h post-hCG. Double labeled: red - H2ax; green - Chek1 (assay MGI:5910517).Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S4B Merge 37 , S4B gH2AX 37
Specimen S4B 48: embryonic day 1.0 (more )
Note: 2-cell embryo 48 h post-hCG. Double labeled: red - H2ax; green - Chek1 (assay MGI:5910517). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S4B gH2AX 48 , S4B Merge 48
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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