Probe:	NZF-2 (Myt1) cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	3B	Expression is in the caudo-ventral region.
TS15: optic vesicle	Present		3B

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	3F	Expression is in the dorsal telencephalon and in basal ganglia.
TS17: forebrain	Present	Regionally restricted	3F	Expression is in the subventricular zone.
TS17: optic cup	Present	Regionally restricted	3F	Expression is in differentiated neurons.
TS17: diencephalon	Present	Regionally restricted	3F	Expression is in the hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3J	Expression is in the cerebral cortex and basal ganglia.
TS19: trigeminal ganglion	Present		3J

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3N	Expression is in the archipallium and basal ganglia.
TS20: cerebral cortex	Present		3N
TS20: hypothalamus	Present		3N
TS20: thalamus	Present		3N

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3R	Expression is in the archipallium and basal ganglia.
TS21: cerebral cortex	Present		3R
TS21: hypothalamus	Present		3R
TS21: thalamus	Present		3R

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present		3V
TS22: hippocampus	Present		3V

Structure	Level	Pattern	Image	Note
TS23: forebrain	Present		3Y
TS23: hippocampus	Present		3Y

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present		4B

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present		4G	Expression was higher at the boundary of the cortical plate and ventricular zone.
TS21: cortical plate	Present		4G	Expression was higher at the boundary of the cortical plate and ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		4L
TS22: cerebral cortex subventricular zone	Present		4L
TS22: cerebral cortex ventricular layer	Present		4L
TS22: cortical plate	Present		4L

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Regionally restricted	5B	Expression is in post-mitotic neurons in the lateral ganglionic eminence.
TS20: telencephalon subventricular zone	Present		5B
TS20: telencephalon ventricular layer	Present	Single cells	5B

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present	Regionally restricted	5F	Expression is in post-mitotic neurons in the medial ganglionic eminence.
TS20: telencephalon subventricular zone	Present		5F
TS20: telencephalon ventricular layer	Present	Single cells	5F

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Weak		5J

Structure	Level	Pattern	Image	Note
TS15: future hindbrain lateral wall	Present	Regionally restricted	6B	Expression is in the basal plate of the hindbrain.
TS15: facio-acoustic preganglion complex	Present		6B
TS15: trigeminal preganglion	Present		6B

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	6F	Expression is in the pons and medulla.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	6J	Expression is in the subventricular zone and there is moderate expression in post-mitotic neurons.
TS20: midbrain	Strong	Regionally restricted	6J	Expression is in the subventricular zone and there is moderate expression in post-mitotic neurons.
TS20: hindbrain ventricular layer	Weak		6J
TS20: midbrain ventricular layer	Weak		6J

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Strong	Regionally restricted	6N	Expression is in the subventricular zone and there is moderate expression in post-mitotic neurons.
TS21: midbrain	Strong	Regionally restricted	6N	Expression is in the subventricular zone and there is moderate expression in post-mitotic neurons.
TS21: hindbrain ventricular layer	Weak		6N
TS21: midbrain ventricular layer	Weak		6N

Structure	Level	Pattern	Image	Note
TS26: cerebellum granule cell layer	Present		6R

Structure	Level	Pattern	Image	Note
TS20: neural retina	Strong	Regionally restricted	7B	Expression is in the inner part of the neural layer and is weak in dispersed cells of the outer part of the nueral layer.

Structure	Level	Pattern	Image	Note
TS21: neural retina	Strong	Regionally restricted	7F	Expression is in the inner part of the neural layer.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Present		7J	Expression is in the inner and outer neuroblastic layers of the neural retina.

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Present	Regionally restricted	7M	Expression is in the basal half.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	8B	Expression is in the earliest born neurons.
TS15: neural tube lateral wall	Present	Regionally restricted	8B	Expression is in the motor neurons of the ventrolateral margin of the spinal cord and in the dispersed cells of alar plate interneurons.
TS15: dorsal root ganglion	Present		8B

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	8J	Expression is in post-mitotic neurons in the subventricular zone with a decreasing ventrolateral gradient.
TS20: spinal cord mantle layer	Weak	Regionally restricted	8J	Expression is in differentiated neurons in the mantle layer with a decreasing ventrolateral gradient.
TS20: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	8N	Expression is in the subventricular zone.
TS21: dorsal root ganglion	Present		8N
TS21: spinal cord mantle layer	Present		8N
TS21: alar columns	Strong	Regionally restricted	8N	Expression is in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: vestibulo-cochlear ganglion	Strong		8R
TS21: facial ganglion	Strong	Scattered	8R
TS21: trigeminal ganglion	Strong	Scattered	8R
TS21: glossopharyngeal ganglion	Present		8R

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present		9B
TS20: spinal cord ventricular layer	Present		9B

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present	Scattered
TS20: olfactory epithelium	Present	Scattered