About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:138816 Plusa B, et al., Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development. 2008 Sep;135(18):3081-91
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5776486
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3B: embryonic day 2.5 (more )
Note: 8-cell embryo; double labeled: white - Nanog; red - Gata6 (assay MGI:5776184).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3B merge , 3B Nanog Expression is seen in the majority of embryos (10 of 11) at this stage.
Specimen 3C: embryonic day 3.5 (more )
Note: 32-cell morula; double labeled: white - Nanog; red - Gata6 (assay MGI:5776184).
Structure Level Pattern Image Note
TS4: compacted morula Strong Widespread 3C merge , 3C Nanog Strong staining is observed in both inside and outside non-dividing cells.
Specimen 3D: embryonic day 3.5 (more )
Note: 33-cell blastocyst; double labeled: white - Nanog; red - Gata6 (assay MGI:5776184).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D merge , 3D Nanog 43 embryos were examined.
TS4: trophectoderm Present 3D merge , 3D Nanog 43 embryos were examined.
Specimen 3E: embryonic day 4.0 (more )
Note: 58-cell blastocyst; double labeled: white - Nanog; red - Gata6 (assay MGI:5776184).
Structure Level Pattern Image Note
TS5: blastocyst Present Regionally restricted 3E merge , 3E Nanog Expression is no longer ubiquitous or nearly ubiquitous. It is mutually exclusive of Gata6.
Specimen S3A: embryonic day 3.5 (more )
Note: 33-cell early blastocyst; double labeled: white - Nanog; red - Cdx2 (assay MGI:5776497).
Structure Level Pattern Image Note
TS4: trophectoderm Present S3A merge , S3A Nanog Expressing cells may also express Cdx2.
TS4: inner cell mass Present S3A Nanog , S3A merge
Specimen S3B: embryonic day 3.5 (more )
Note: 32-cell early blastocyst; double labeled: white - Nanog; red - Cdx2 (assay MGI:5776497).
Structure Level Pattern Image Note
TS4: trophectoderm Present S3B merge , S3B Nanog Expressing cells may also express Cdx2.
TS4: inner cell mass Present S3B merge , S3B Nanog
Specimen S3C: embryonic day 4.0 (more )
Note: 60-cell expanded blastocyst; double labeled: white - Nanog; red - Cdx2 (assay MGI:5776497).
Structure Level Pattern Image Note
TS5: inner cell mass Present S3C Nanog , S3C merge
TS5: trophectoderm Absent S3C merge , S3C Nanog
Specimen 4A: embryonic day 3.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 32-cell blastocyst; triple labeled: white - Nanog ; green - Pdgfra reporter (assay MGI:5776158); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 4A merge , 4A Nanog
Specimen 4B: embryonic day 4.0; Pdgfratm11(EGFP)Sor/? (more )
Note: 64-cell blastocyst; triple labeled: white - Nanog ; green - Pdgfra reporter (assay MGI:5776158); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS5: blastocyst Present Single cells 4B Nanog , 4B merge There is a salt and pepper distribution of expression.
Specimen 4C: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 115-cell blastocyst; triple labeled: white - Nanog ; green - Pdgfra reporter (assay MGI:5776158); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: epiblast Present 4C merge , 4C Nanog
Specimen 4D: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 118-cell blastocyst; triple labeled: white - Nanog ; green - Pdgfra reporter (assay MGI:5776158); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: epiblast Present 4D Nanog , 4D merge
TS6: primitive endoderm Absent 4D Nanog , 4D merge
Specimen 5A: embryonic day 4.0 (more )
Note: 65-cell blastocyst; double labeled: white - Nanog; red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS5: inner cell mass Present Single cells 5A There is a salt and pepper distribution of expression.
Specimen 5B: embryonic day 4.0 (more )
Note: 72-cell blastocyst; double labeled: white - Nanog; red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS5: inner cell mass Present Single cells 5B There is a salt and pepper distribution of expression.
Specimen 5C: embryonic day 4.5 (more )
Note: 108-cell blastocyst; double labeled: white - Nanog; red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: epiblast Present 5C At this stage approximately half the embryos exhibit partial or complete segregation of expression to this layer; the other embryos exhibit the salt and pepper distribution pattern of expression seen at earlier stages.
Specimen 5D: embryonic day 4.5 (more )
Note: 115-cell blastocyst; double labeled: white - Nanog; red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: epiblast Present 5D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory