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Gene Expression Data
Assay Details
Assay
Reference: J:138816 Plusa B, et al., Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development. 2008 Sep;135(18):3081-91
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5776158
Gene symbol: Pdgfra
Gene name: platelet derived growth factor receptor, alpha polypeptide
Reporter: GFP
Antibody: anti-GFP (Invitrogen)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Heterozygous and homozygous embryos were indistinguishable.
Results
Specimen 1B: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: Double labeled: green - Pdgfra reporter; red - Pdgfra protein (assay MGI:5776173).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 1B GFP , 1B merge Expression is localized to the primitive endoderm layer of the blastocyst; reporter expression co-localizes with the endogenous protein expression.
Specimen 1C: embryonic day 4.0; Pdgfratm11(EGFP)Sor/? (more )
Note: Double labeled: green - Pdgfra reporter; red - Gata6 (assay MGI:5776184).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 1C GFP , 1C merge Expression is localized to the primitive endoderm layer of the blastocyst; double labeling demonstrates that reporter expression co-localizes with expression of a marker of this layer.
Specimen 1D: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: Double labeled: green - Pdgfra reporter; red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 1D GFP , 1D merge Expression is localized to the primitive endoderm layer of the blastocyst; double labeling demonstrates that reporter expression co-localizes with expression of a marker of this layer.
Specimen 1E: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: Double labeled: green - Pdgfra reporter; red - Dab2 (assay MGI:5776185).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 1E GFP , 1E merge Expression is localized to the primitive endoderm layer of the blastocyst; double labeling demonstrates that reporter expression co-localizes with expression of a marker of this layer.
Specimen S1A: embryonic day 3.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 32-cell morula; double labeled: green - Pdgfra reporter; red - Pdgfra protein (assay MGI:5776173).
Structure Level Pattern Image Note
TS4: compacted morula Present Single cells S1A sxn merge , S1A GFP , S1A 3D merge Reporter expression preceeds observable protein expression; the protein would be distributed in the plasma membrane.
Specimen S1B: embryonic day 3.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 33-cell blastocyst; double labeled: green - Pdgfra reporter; red - Pdgfra protein (assay MGI:5776173).
Structure Level Pattern Image Note
TS4: inner cell mass Present Single cells S1B GFP , S1B sxn merge , S1B 3D merge The endogenous protein is weakly expressed in a subset of the reporter expressing cells; it is localized in the plasma membrane.
Specimen S1C: embryonic day 4.0; Pdgfratm11(EGFP)Sor/? (more )
Note: 61-cell embryo; double labeled: green - Pdgfra reporter; red - Pdgfra protein (assay MGI:5776173).
Structure Level Pattern Image Note
TS5: inner cell mass Present Single cells S1C 3D merge , S1C sxn merge , S1C GFP Reporter expression co-localizes with endogenous protein expression.
Specimen 3A: embryonic day 3.0; Pdgfratm11(EGFP)Sor/? (more )
Note: 16-cell embryo; double labeled: green - Pdgfra reporter; red - Gata6 (assay MGI:5776184).
Structure Level Pattern Image Note
TS3: compacted morula Present Single cells 3A merge , 3A GFP Expression is seen in a subset of cells.
Specimen 4A: embryonic day 3.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 32-cell blastocyst; triple labeled: green - Pdgfra reporter; white - Nanog (assay MGI:5776486); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 4A merge , 4A GFP
Specimen 4B: embryonic day 4.0; Pdgfratm11(EGFP)Sor/? (more )
Note: 64-cell blastocyst; triple labeled: green - Pdgfra reporter; white - Nanog (assay MGI:5776486); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS5: blastocyst Present Single cells 4B merge , 4B GFP There is a salt and pepper distribution of expression.
Specimen 4C: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 115-cell blastocyst; triple labeled: green - Pdgfra reporter; white - Nanog (assay MGI:5776486); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 4C GFP , 4C merge
Specimen 4D: embryonic day 4.5; Pdgfratm11(EGFP)Sor/? (more )
Note: 118-cell blastocyst; triple labeled: green - Pdgfra reporter; white - Nanog (assay MGI:5776486); red - Gata4 (assay MGI:5776181).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 4D merge , 4D GFP
TS6: epiblast Absent 4D merge , 4D GFP
Specimen S6B: embryonic day 4.0; Pdgfratm11(EGFP)Sor/? (more )
Note: 76-cell blastocyst; double labeled: green - Pdgfra reporter; red - cleaved Casp3 (assay MGI:5776505).
Structure Level Pattern Image Note
TS5: inner cell mass Present Single cells S6B triple label , S6B double label
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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