Antibody:	anti-Pdgfra
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Heterozygous and homozygous embryos were indistinguishable.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	1A merge , 1A PDGFra	Expression is localized to the primitive endoderm layer of the blastocyst; it co-localized with another expression marker for this layer.

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		1B GFP , 1B PDGFRa	Expression is localized to the primitive endoderm layer of the blastocyst; endogenous protein expression co-localizes with reporter expression.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Absent		S1A 3D merge , S1A sxn merge , S1A Pdgfra	Reporter expression preceeds observable protein expression.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Single cells	S1B sxn merge , S1B Pdgfra , S1B 3D merge	The endogenous protein is weakly expressed in a subset of the reporter expressing cells.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Single cells	S1C sxn merge , S1C 3D merge , S1C Pdgfra	Endogenous protein expression co-localizes with reporter expression.