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Gene Expression Data
Assay Details
Assay
Reference: J:171281 Nakagawa S, et al., Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 2011 Apr 4;193(1):31-9
Assay type: RNA in situ
MGI Accession ID: MGI:5770404
Gene symbol: Neat1
Gene name: nuclear paraspeckle assembly transcript 1 (non-protein coding)
Probe: NEAT1_2 probe
Probe preparation: labelled with fluorescein
Results
Specimen 2C (3rd row): postnatal adult (more )
Note: Surface epithelial cells. Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: stomach epithelium Present Regionally restricted 2C NEAT1_2 (3rd row) , 2C merge (3rd row) Expression concentrated in discrete large foci in surface epithelial cells.
Specimen 2D (3rd row): postnatal adult (more )
Note: Parietal cells. Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: stomach epithelium Weak Regionally restricted 2D NEAT1_2 (3rd row) , 2D merge (3rd row) Gastric cells deeper in the epithelium, such as parietal cells, lack strong expression. Occasional strongly expressing cells in the deep layer.
Specimen 4D WT esophagus: postnatal adult (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: esophagus epithelium Weak Regionally restricted 4D WT esophagus NEAT1_2 , 4D WT esophagus (merge)
Specimen 4D KO esophagus: postnatal adult; Neat1tm1.1Shna/Neat1tm1.1Shna (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: esophagus epithelium Absent 4D KO esophagus (merge) , 4D KO esophagus NEAT1_2
Specimen 4D WT forestomach: postnatal adult (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: stomach proventricular region epithelium Weak Regionally restricted 4D WT forestomach NEAT1_2 , 4D WT forestomach (merge)
Specimen 4D KO forestomach: postnatal adult; Neat1tm1.1Shna/Neat1tm1.1Shna (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: stomach proventricular region epithelium Absent 4D KO forestomach NEAT1_2 , 4D KO forestomach (merge)
Specimen 4D WT s. epithelium: postnatal adult (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: stomach epithelium Weak Regionally restricted 4D WT s. epithelium NEAT1_2 , 4D WT s. epitehlium (merge)
Specimen 4D KO s. epithelium: postnatal adult; Neat1tm1.1Shna/Neat1tm1.1Shna (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: stomach epithelium Absent 4D KO s. epithelium NEAT1_2 , 4D KO s. epithelium (merge)
Specimen S2A (3rd row): postnatal adult (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: liver Present S2A merge (3rd row) , S2A NEAT1_2 (3rd row) Authors state expression levels were highly variable between in animals in certain tissues such as liver.
Specimen S2B (3rd row): postnatal adult (more )
Note: Double labeled: green - NEAT1 (long probe); pink - Sfpq (PSF; assay MGI:5770412).
Structure Level Pattern Image Note
TS28: liver Present S2B NEAT1_2 (3rd row) , S2B merge (3rd row) Authors state expression levels were highly variable between in animals in certain tissues such as liver.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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