Neat1^tm1.1Shna
Targeted Allele Detail

Summary

• Symbol: Neat1^tm1.1Shna
• Name: nuclear paraspeckle assembly transcript 1 (non-protein coding); targeted mutation 1.1, Shinichi Nakagawa
• MGI ID: MGI:4949933
• Synonyms: NEAT1^lacZ
• Gene: Neat1 Location: Chr19:5874736-5895508 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Neat1^tm1.1Shna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171281
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A lacZ and FRT flanked neo cassette was inserted immediately downstream of the transcription start site via homologous recombination. Flp mediated recombination removed the neo cassette. Quantitative PCR analysis indicates that expression of the short transcript is decreased to about 6% of wild-type and expression of the long transcript is absent in MEFs from homozygous mice. (J:171281)

Expression

In Mice Carrying this Mutation:	17 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Neat1 Mutation: 222 strains or lines available

References

• Original: J:171281 Nakagawa S, et al., Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 2011 Apr 4;193(1):31-9
• All: 13 reference(s)