Gene Expression Data
Assay Details
Assay
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Reference:
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J:174119
Glickstein SB, et al., Differences in cyclin D2 and D1 protein expression distinguish forebrain progenitor subsets. Cereb Cortex. 2007 Mar;17(3):632-42
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5751977
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Gene symbol:
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Ccnd1
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Gene name:
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cyclin D1
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Results
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Specimen
3G/I:
embryonic day 14.5
(more )
Note: Double labeled: green - Ccnd1; red - Ccnd2 (assay MGI:5751978).
Specimen
3G/I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Ccnd1; red - Ccnd2 (assay MGI:5751978).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: ganglionic eminence |
Present |
Regionally restricted |
3G
,
3I
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In the ventricular zone, most progenitors expressed either Ccnd1 or Ccnd2, but a few cells expressed both cyclins.
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Specimen
3J/L:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ccnd2tm1Wbg/Ccnd2tm1Wbg
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Ccnd1; red - Ccnd2 (assay MGI:5751978).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: ganglionic eminence |
Present |
Regionally restricted |
3J
,
3L
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In the ventricular zone, most progenitors expressed either Ccnd1 or Ccnd2, but a few cells expressed both cyclins.
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Specimen
8J:
postnatal day 60
(more )
Note: Double labeled with BrdU (red).
Specimen
8J:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
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Double labeled with BrdU (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: dentate gyrus |
Present |
|
8J
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Some immunoreactive cells were also labeled with BrdU.
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Specimen
S4B/C:
embryonic day 12.5
(more )
Note: Double labeled: green - Ccnd1; red - Ccnd2 (assay MGI:5751978).
Specimen
S4B/C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: green - Ccnd1; red - Ccnd2 (assay MGI:5751978).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: medial ganglionic eminence |
Present |
Regionally restricted |
S4C
,
S4B
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In the ventricular zone of the medial ganglionic eminence, most progenitors expressed either Ccnd1 or Ccnd2, but a few cells expressed both cyclins.
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Specimen
S4H/I:
embryonic day 12.5
(more )
Note: Double labeled with BrdU (red).
Specimen
S4H/I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled with BrdU (red).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: ganglionic eminence |
Present |
Regionally restricted |
S4I
,
S4H
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Many immunolabeled cells were also labeled with BrdU.
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