Antibody:

Anti-Cyclin D1 (SP4 RM-9104)

Structure	Level	Pattern	Image	Note
TS21: lens	Present		1C
TS21: retina	Present	Widespread	1C

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present	Widespread	1G

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Absent		1H

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present	Regionally restricted	1K	Expression was in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Absent		1L

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1O	Expression was detected in the ventricular zone of the hippocampal formation.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Absent		1P

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		2B

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		2C	Expression was similar to that observed in wild type.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present	Uniform	2E	The density of immunoreactive cells was reduced compared to E12.5.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		2F	Expression was similar to that observed in wild type.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		2H
TS25: cerebral cortex ventricular zone	Present	Non-Uniform	2H

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Regionally restricted	3B	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.
TS20: medial ganglionic eminence	Present	Regionally restricted	3B	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Regionally restricted	3C	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.
TS20: medial ganglionic eminence	Present	Regionally restricted	3C	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Present	Regionally restricted	3E	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.
TS22: medial ganglionic eminence	Present	Regionally restricted	3E	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Present	Regionally restricted	3F	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.
TS22: medial ganglionic eminence	Present	Regionally restricted	3F	Expression was robust throughout the ventricular zone and also labeled some nuclei and processes in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Present	Regionally restricted	4B , 4D	Expression was detected in the striatal ventricular zone immediately adjacent to the ventricular wall. Immunoreactive neurons were scattered in the striatum.
TS26: caudate-putamen	Present	Regionally restricted	4B , 4D	Expression was detected in cells and striatal processes in the caudato-putamen.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5B	Expression was detected in the hippocampal ventricular zone. No immunolabeled cells were observed in the marginal zone or in the region just rostral to the fimbrial glioepithelium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5C	Expression in the hippocampal ventricular zone of the mutant was comparable to wild type.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	5E	Expression was detected in the ammonic ventricular zone. Only a few immunoreactive neurons were detected in the ammonic marginal zone.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	5F	Expression in the ammonic ventricular zone of the mutant was similar to wild type.
TS22: telencephalon	Present	Regionally restricted	5F	Immunolabled cells were located in the mutant cortical hem unlike in the wild type.

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		6B
TS26: hippocampal formation	Weak	Regionally restricted	6B	Expression was dramatically reduced in the dentate migratory stream compared to E17.5.
TS26: hippocampus	Weak	Regionally restricted	6B	Expression was dramatically reduced in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		6E
TS26: hippocampus	Weak	Regionally restricted	6E	Expression was greatly reduced in the ventricular zone of the temporal hippocampus compared to the septal hippocampus.
TS26: hippocampal formation	Weak	Regionally restricted	6E	Expression was dramatically reduced in the dentate migratory stream compared to E17.5.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Scattered	7D , 7B
TS28: dentate gyrus granule cell layer	Strong		7B , 7D

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		7F	Expression in the mutant was equivalent to the wild type.

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present	Scattered	8D
TS28: CA1 pyramidal cell layer	Strong		8D

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present	Scattered	8E
TS28: CA1 pyramidal cell layer	Strong		8E

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Absent		8F

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Scattered	8G	Expression was in cells scattered in all layers of the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Scattered	8H	Expression was in cells scattered in all layers of the dentate gyrus as in wild type.

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Scattered	S2C

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		S2F	Compared to P1, immunolabeling was much more numerous by P7.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		S3F	Expression was abundant in presumed postmitotic neurons.
TS28: cortical layer III	Present		S3F	Expression was abundant in presumed postmitotic neurons.
TS28: cortical layer V	Present		S3F	Expression was abundant in presumed postmitotic neurons.
TS28: cortical layer VI	Present		S3F	Expression was abundant in presumed postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		S3G	Expression was abundant in presumed postmitotic neurons.
TS28: cortical layer III	Present		S3G	Expression was abundant in presumed postmitotic neurons.
TS28: cortical layer V	Present		S3G	Expression was abundant in presumed postmitotic neurons.
TS28: cortical layer VI	Present		S3G	Expression was abundant in presumed postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Absent		S3H
TS28: cortical layer III	Absent		S3H
TS28: cortical layer V	Absent		S3H
TS28: cortical layer VI	Absent		S3H

Structure	Level	Pattern	Image	Note
TS20: caudal ganglionic eminence	Present	Regionally restricted	S5B	Expression in the ventricular zone was homogeneously distributed and was scattered in subventricular zone neurons. Labeled processes were noted in the lateral caudal ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: caudal ganglionic eminence	Present	Regionally restricted	S5D	Expression in the ventricular zone was evenly packed and was scattered in subventricular zone neurons. Labeled processes were noted in the lateral caudal ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Present	Regionally restricted	S6B	Expression was detected in the strip of tissue immediately adjacent to the ventricle and in scattered neurons.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Weak	Regionally restricted	S6D	The ventricular zone/subventricular zone labeling decreased.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present	Scattered

Structure	Level	Pattern	Image	Note
TS25: hippocampal formation	Present	Regionally restricted	S7A	Expression was detected in the ventricular zone and in the secondary and tertiary matrices.