Probe:	Dach cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: brain	Present		5A E10.5
TS17: eye	Present		5A E10.5
TS17: glossopharyngeal ganglion	Present		5A E10.5
TS17: limb	Present		5A E10.5
TS17: neural tube	Present		5A E10.5
TS17: trigeminal ganglion	Present		5A E10.5
TS17: vagus ganglion	Present		5A E10.5
TS17: axial skeleton thoracic region	Present	Regionally restricted	5A E10.5	Expression is in rib primordia.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		5A E11.5
TS19: eye	Present		5A E11.5
TS19: glossopharyngeal ganglion	Present		5A E11.5
TS19: limb	Present		5A E11.5
TS19: neural tube	Present		5A E11.5
TS19: rib pre-cartilage condensation	Present		5A E11.5
TS19: trigeminal ganglion	Present		5A E11.5
TS19: vagus ganglion	Present		5A E11.5

Structure	Level	Pattern	Image	Note
TS20: brain	Present		5A E12.5
TS20: eye	Present		5A E12.5
TS20: glossopharyngeal ganglion	Present		5A E12.5
TS20: limb	Present		5A E12.5
TS20: spinal cord	Present		5A E12.5
TS20: trigeminal ganglion	Present		5A E12.5
TS20: vagus ganglion	Present		5A E12.5

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		5B
TS19: neural tube	Present		5B

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present	Regionally restricted	5C E10.5 top	There is an anterior region of expression that extends to the center of the limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	5C E10.5 bottom	There is an anterior region of expression that extends to the center of the limb bud.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	5C E11.5 top	Expresion is at the periphery.
TS19: footplate mesenchyme	Present	Regionally restricted	5C E11.5 top	Expression is in the mesenchyme beneath the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	5C E11.5 bottom	Expresion is at the periphery.
TS19: handplate mesenchyme	Present	Regionally restricted	5C E11.5 bottom	Expression is in the mesenchyme beneath the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	5C E12.5 top	Expresion is at the periphery.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	5C E12.5 bottom	Expresion is at the periphery.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit mesenchyme	Present	Regionally restricted	5C E13.5 top	Expression is at the tips.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present	Regionally restricted	5C E13.5 bottom	Expression is at the tips.

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present		5D

Structure	Level	Pattern	Image	Note
TS19: rib pre-cartilage condensation	Present	Punctate	5E

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5F	Including the olfactory bulb.
TS19: diencephalon	Absent		5F

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present		5G
TS19: head mesenchyme	Present	Regionally restricted	5G	Expression is in the mesenchyme surrounding the eye.
TS19: pigmented retinal epithelium	Absent		5G

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Present	Regionally restricted	5I	Expression is within the dorsal, lateral, and ventral mantle layer.
TS19: neural tube ventricular layer	Present	Regionally restricted	5I	Expression is dorsal.
TS19: dorsal root ganglion	Present		5I

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present		6A

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present		6B	Expression in this mutant is the same as in wild-type.