Probe:	Sox10 probe11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		1G	Expression is in nascent neural crest cells.

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Present		1H
TS13: future spinal cord neural crest	Present		1H

Structure	Level	Pattern	Image	Note
TS13: future brain neural crest	Strong		1I
TS13: future spinal cord neural crest	Present		1I

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		1J

Structure	Level	Pattern	Image	Note
TS13: neural crest	Strong		1K	The axial extent of neural crest staining was expanded caudally in this mutant.

Structure	Level	Pattern	Image	Note
TS13: neural crest	Strong		1L	The axial extent of neural crest staining was expanded caudally in this mutant.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural crest	Present	Regionally restricted	3E	Expression is in neural crest cells at the level of 6th and 8th somites and extends rostrally.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3F wild-type
TS15: neural crest	Present		3F wild-type

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3F Nog-/-	Expression in migrating and non-migrating neural crest extends more caudally in this mutant.
TS15: neural crest	Present		3F Nog-/-	Expression in migrating and non-migrating neural crest extends more caudally in this mutant.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3G wild-type
TS15: neural crest	Present		3G wild-type

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		3G Chrd-/-;Nog-/-	Axial levels of neural crest cell specification and migration are displaced further caudally in mutants.
TS15: neural crest	Present		3G Chrd-/-;Nog-/-	Axial levels of neural crest cell specification and migration are displaced further caudally in mutants.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		4D
TS15: neural crest	Present		4D

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Strong		4E	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube.
TS15: neural crest	Strong		4E	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Strong		4F	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube.
TS15: neural crest	Strong		4F	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		4G
TS15: neural crest	Present		4G

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	4H	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube and around the midgut region.
TS15: neural crest	Present	Regionally restricted	4H	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube and around the midgut region.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	4I	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube.
TS15: neural crest	Present	Regionally restricted	4I	There is an increase in neural crest cells. Large ectopic aggregations of neural crest cells are seen superficial to the dorsal neural tube.