Probe:	LRH-1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: urogenital ridge	Absent

Structure	Level	Pattern	Image	Note
TS17: urogenital ridge	Absent		1B LRH-1 E10.5 , 1A LRH-1 E10.5

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		1B LRH-1 E11.5 , 1A LRH-1 E11.5	Expression was detected in the gonad, but not the adrenal primordium. Expression was in a subset of germ cells and in somatic cells surrounding the positive germ cells.
TS19: primordial germ cell of gonad	Present	Regionally restricted	1A LRH-1 E11.5 , 1B LRH-1 E11.5	Expression was detected in a subset of germ cells.
TS19: urogenital ridge	Present	Regionally restricted	1B LRH-1 E11.5 , 1A LRH-1 E11.5	Sparse signal was noted in the genital ridge epithelium.

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Widespread	1B LRH-1 Ovary E13.5 , 1A LRH-1 Ovary E13.5	Expression was detected throughout the ovary, but absent in the overlying epithelium. Signal was localized to germ cells within germline cysts and to somatic cells in contact with germ cells.
TS21: germ cell of ovary	Present		1A LRH-1 Ovary E13.5 , 1B LRH-1 Ovary E13.5
TS21: pancreas	Strong		1A LRH-1 Ovary E13.5

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present	Regionally restricted	1B LRH-1 Testis E13.5 , 1A LRH-1 Testis E13.5	Expression was detected in germ cells and pre-Sertoli cells.
TS21: germ cell of testis	Present		1B LRH-1 Testis E13.5 , 1A LRH-1 Testis E13.5

Structure	Level	Pattern	Image	Note
TS23: ovary	Weak		1A LRH-1 Ovary E15.5

Structure	Level	Pattern	Image	Note
TS23: testis	Weak		1A LRH-1 Testis E15.5
TS23: intestine	Present		1A LRH-1 Testis E15.5

Structure	Level	Pattern	Image	Note
TS23: pancreas	Strong

Structure	Level	Pattern	Image	Note
TS27: testis	Weak		2_LRH-1 P2
TS27: germ cell of testis	Present		2_LRH-1 P2 , 2_LRH-1 P2 brightfield

Structure	Level	Pattern	Image	Note
TS28: testis	Trace		2_LRH-1 P8

Structure	Level	Pattern	Image	Note
TS28: epithelium of epididymis	Absent		2_LRH-1 P24
TS28: testis	Absent		2_LRH-1 P24

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		2_LRH-1 Ad

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		3A

Structure	Level	Pattern	Image	Note
TS27: oocyte	Present		3C left	Expression was detected in naked oocytes, lacking granulosa cells in the cortical region of the ovary. Naked oocytes with no signal were also noted in the cortex.

Structure	Level	Pattern	Image	Note
TS27: ovary primordial follicle	Present		3C right	Expression was detected in the primordial follicles in the medullary region. Both the oocyte and the surrounding granulosa cells were positive.
TS27: follicle cell of primordial follicle	Present		3C right
TS27: oocyte of primordial follicle	Present		3C right
TS27: ovary primary follicle	Present		3C right	A small number of single-layer primary follicles in the medullary region displayed signal primarily in the granulosa cells and to a lesser extent in the oocyte.
TS27: follicle cell of primary follicle	Present		3C right
TS27: oocyte of primary follicle	Present		3C right
TS27: ovary	Present	Regionally restricted	3C right	In this section of the medullary region of the ovary, signal was not detected in naked oocytes, lacking granulosa cells.

Structure	Level	Pattern	Image	Note
TS27: ovary	Present	Regionally restricted	4_LRH-1 P2	The majority of signal was in the medullary portion.

Structure	Level	Pattern	Image	Note
TS28: follicle cell of primary follicle	Present		4_LRH-1 P8	Single-layer primary follicles exhibited signal in both oocytes and granulosa cells. Multilayer primary follicles had signal only in granulosa cells.
TS28: oocyte of primary follicle	Present		4_LRH-1 P8	Single-layer primary follicles exhibited signal in both oocytes and granulosa cells. Multilayer primary follicles had signal only in granulosa cells.
TS28: ovary follicle	Present	Regionally restricted	4_LRH-1 P8	Multilayer primary and larger follicles had signal only in granulosa cells.
TS28: ovary atretic follicle	Weak		4_LRH-1 P8

Structure	Level	Pattern	Image	Note
TS28: follicle cell of primary follicle	Present		4_LRH-1 P24	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: follicle cell of secondary follicle	Present		4_LRH-1 P24	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: follicle cell of tertiary follicle	Present		4_LRH-1 P24	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: ovary antral follicle	Present		4_LRH-1 P24	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: ovary atretic follicle	Weak		4_LRH-1 P24
TS28: oviduct	Absent		4_LRH-1 P24

Structure	Level	Pattern	Image	Note
TS28: follicle cell of primary follicle	Present		4_LRH-1 Ad	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: follicle cell of secondary follicle	Present		4_LRH-1 Ad	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: follicle cell of tertiary follicle	Present		4_LRH-1 Ad	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: ovary antral follicle	Present		4_LRH-1 Ad	Expression was detected in granulosa cells of follicles from primary to antral stage.
TS28: ovary atretic follicle	Weak		4_LRH-1 Ad
TS28: oviduct	Absent		4_LRH-1 Ad