Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	2A	Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	2B	There was no difference in expression in melanoblasts in the mutant at E11.5.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	2E , 2C	Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	2D , 2F	There was a striking reduction of positive melanoblasts in the trunk laterally between both limbs, but not in the tail or head region.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	2G	Expression was detected in melanocytes.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	2H	Positive melanocytes were significantly reduced.

Structure	Level	Pattern	Image	Note
TS23: skin	Present	Regionally restricted	2I	Expression was detected in melanocytes.

Structure	Level	Pattern	Image	Note
TS23: skin	Present	Regionally restricted	2J	Positive melanocytes were significantly reduced.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	2M , 2K	Expression was detected in melanocytes.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	2N , 2L	Positive melanocytes were significantly reduced.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present	Clusters	2O	Positive melanoblasts were detected in large clusters marking the hair follicles.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Absent		2P	There was a dramatic reduction of follicle-associated melanoblast clusters.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present	Regionally restricted	2Q	Expression was detected in melanoblasts.
TS24: epidermis	Present	Regionally restricted	2Q	Expression was detected in melanoblasts.
TS24: hair follicle	Present	Regionally restricted	2Q	Expression was detected in melanoblasts.
TS24: peripheral nervous system	Present		2Q

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Absent		2R
TS24: epidermis	Present	Regionally restricted	2R	Positive melanoblasts were significantly reduced in the epidermis.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted		Expression was detected in melanoblasts.
TS23: hair follicle	Present	Regionally restricted		Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Absent
TS23: epidermis	Present	Regionally restricted		Positive melanoblasts were significantly reduced in the epidermis.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Regionally restricted	S1A , S1C	Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Regionally restricted	S1D , S1B	The reduction of positive melanoblasts in the trunk continued at E13.5.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	S1E , S1G	Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	S1H , S1F	The reduction of positive melanoblasts in the trunk continued at E14.5.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	S1I	Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	S1J	The reduction of positive melanoblasts in the trunk continued at E16.5.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	S1K	Expression was detected in melanoblasts.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted	S1L	The reduction of positive melanoblasts in the trunk continued at E16.5.