Probe:	Mash1 cDNA10
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2C'	Expression was seen in the central part of the olfactory bulb, but not in the cerebral cortex (signal in figure was artifactual).
TS19: diencephalon	Present	Regionally restricted	2C'	Expression was strong in the preoptic region, but there was no signal in the eminentia thalami. The pretectal alar plate, the basal plate portions of prosomeres 1-3, and the zona limitans intrathalamica had expression also.
TS19: midbrain	Present	Regionally restricted	2C'	Expression was detected in the tegmentum.
TS19: cerebellum primordium	Present		2C'
TS19: myelencephalon alar plate	Present	Regionally restricted	2C'	Expression was detected in a longitudinal stripe.
TS19: myelencephalon basal plate	Present	Regionally restricted	2C'	Expression was detected in a longitudinal stripe.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon alar plate	Present	Regionally restricted	4G	Expression was detected in a longitudinal stripe.
TS19: myelencephalon basal plate	Present	Regionally restricted	4G	Expression was detected in a longitudinal stripe.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon alar plate	Present	Regionally restricted	4H	Expression was detected in a longitudinal stripe.
TS19: myelencephalon basal plate	Present	Regionally restricted	4H	Expression was detected in a longitudinal stripe.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon alar plate	Present	Regionally restricted	4I	Expression was detected in a longitudinal stripe.
TS19: myelencephalon basal plate	Present	Regionally restricted	4I	Expression was detected in a longitudinal stripe.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5A	Expression was seen in the central part of the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5B
TS20: telencephalon	Strong	Regionally restricted	5B	Strong expression was detected in the septum.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5C
TS20: medial ganglionic eminence	Strong		5C
TS20: telencephalon	Strong	Regionally restricted	5C	Strong expression was detected in the septum.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5D
TS20: medial ganglionic eminence	Strong		5D
TS20: preoptic area	Strong		5D
TS20: hypothalamus	Present	Regionally restricted	5D	Expression was in the basal plate of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5E
TS20: medial ganglionic eminence	Strong		5E
TS20: zona limitans intrathalamica	Strong		5E
TS20: hypothalamus	Present	Regionally restricted	5E	Expression was in the basal plate of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5F
TS20: medial ganglionic eminence	Strong		5F
TS20: preoptic area	Strong		5F
TS20: hypothalamus	Present	Regionally restricted	5F	Expression was in the basal plate of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5G
TS20: medial ganglionic eminence	Strong		5G
TS20: preoptic area	Strong		5G
TS20: hypothalamus	Present	Regionally restricted	5G	Expression was in the basal plate of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5H
TS20: medial ganglionic eminence	Strong		5H
TS20: hypothalamus	Present	Regionally restricted	5H	Expression was in the basal plate of the hypothalamus, but was not in the alar hypothalamus.
TS20: thalamus	Strong	Regionally restricted	5H	Expression was strong in the ventral thalamus, but no signal was in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong		5I
TS20: medial ganglionic eminence	Strong		5I
TS20: thalamus	Strong	Regionally restricted	5I	Expression was strong in the ventral thalamus, but no signal was in the dorsal thalamus.
TS20: hypothalamus	Present	Regionally restricted	5I	Expression was in the basal plate of the hypothalamus, but was not in the alar hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: caudal ganglionic eminence	Strong		5J
TS20: thalamus	Strong	Regionally restricted	5J	Expression was strong in the ventral thalamus, but no signal was in the dorsal thalamus. The zona limitans intrathalamica also had expression.
TS20: hypothalamus	Present	Regionally restricted	5J	Expression was in the basal plate of the hypothalamus, but was not in the alar hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: caudal ganglionic eminence	Strong		5K
TS20: thalamus	Strong	Regionally restricted	5K	Expression was strong in the ventral thalamus, but no signal was in the dorsal thalamus. The zona limitans intrathalamica also had expression.
TS20: hypothalamus	Present	Regionally restricted	5K	Expression was in the basal plate of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		5L

Structure	Level	Pattern	Image	Note
TS21: midbrain	Strong	Regionally restricted	5M	Expression is strong in the superior and inferior colliculus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6A	Expression was detected in the basal plate of the hypothalamus, but no signal was seen in the dorsal thalamus. The pretectal alar plate and basal plate portions of prosomeres 1-3 had expression also.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	6C	Expression is strong in the superior and inferior colliculus. A strong stripe of expression extends from the inferior colliculus toward the cerebellum. Expression was also detected in the tegmentum.
TS19: cerebellum primordium	Present		6C

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	6D	Expression is strong in the superior and inferior colliculus. A strong stripe of expression extends from the inferior colliculus toward the cerebellum.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Strong		6E
TS21: thalamus	Strong	Regionally restricted	6E	Expression was strong in the ventral thalamus, but no signal was detected in the dorsal thalamus.
TS21: hypothalamus	Present	Regionally restricted	6E	Expression was detected in the basal hypothalamus, but was not in the alar hypothalamus.