Probe:	Ngn2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	1_whole mount	Expression was strong in the dorsal pallium.
TS19: midbrain	Strong	Regionally restricted	1_whole mount	Expression was strong in the pretectum and superior colliculus. More faintly, expression in the basal plate of the mesencephalic tegmentum was observed.
TS19: future spinal cord	Strong		1_whole mount
TS19: diencephalon	Weak	Regionally restricted	1_whole mount	Expression in the prosomeres 1-3 was faint in whole mount.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1_inset	Expression was detected in the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	2B'	Expression was detected in prosomeres 1-3.
TS20: zona limitans intrathalamica	Present		2B'
TS20: preoptic area	Absent		2B'
TS20: midbrain	Present	Regionally restricted	2B'	Expression was detected in the basal plate of the mesencephalic tegmentum and in the ventral aspect of the superior and inferior colliculus. There was a gap between the expression domains in the anterior and posterior pretectum.
TS20: ganglionic eminence	Absent		2B'
TS20: midbrain-hindbrain junction	Absent		2B'
TS20: telencephalon	Present	Regionally restricted	2B'	Expression was detected in the cortex, but no signal was seen in the septum.
TS20: thalamus	Present	Regionally restricted	2B'	Expression was detected in the dorsal thalamic alar plate. No expression was in the ventral thalamic alar plate.
TS20: hypothalamus	Present	Regionally restricted	2B'	Expression was detected in the anterior hypothalamus.
TS20: medulla oblongata	Present	Regionally restricted	2B'	Two longitudinal stripes of expression were detected in the medulla oblongata.
TS20: cerebellum primordium	Present		2B'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3A	Expression was detected in olfactory bulb.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Widespread	3B
TS20: telencephalon	Present	Regionally restricted	3B	Expression was present in all parts of the cortex. No signal was in the septum.
TS20: ganglionic eminence	Absent		3B

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Absent		3C

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Absent		3D
TS20: diencephalon	Present	Regionally restricted	3D	Expression was in the eminentia thalami.
TS20: preoptic area	Absent		3D
TS20: thalamus	Present	Regionally restricted	3D	Expression was detected in the dorsal thalamic alar plate. No expression was in the ventral thalamic alar plate.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Absent		3E
TS20: thalamus	Present	Regionally restricted	3E	Expression was detected in the dorsal thalamic alar plate. No expression was in the ventral thalamic alar plate.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Absent		3F
TS20: thalamus	Present	Regionally restricted	3F	Expression was detected in the dorsal thalamic alar plate. No expression was in the ventral thalamic alar plate.
TS20: midbrain	Present	Regionally restricted	3F	Expression in the pretectum was less dense and more remote from the ventricle compared to the thalamus.
TS20: zona limitans intrathalamica	Present		3F
TS20: hypothalamus	Present	Regionally restricted	3F	Expression was detected in the anterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3G	Expression was detected in the dorsal thalamic alar plate. No expression was in the ventral thalamic alar plate.
TS20: midbrain	Present	Regionally restricted	3G	Expression in the pretectum was less dense and more remote from the ventricle compared to the thalamus.
TS20: zona limitans intrathalamica	Present		3G

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3H	Expression was detected in the dorsal thalamic alar plate.
TS20: midbrain	Present	Regionally restricted	3H	Expression in the pretectum was less dense and more remote from the ventricle compared to the thalamus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	3I	Expression was strong in basal prosomeres 1-3.
TS20: midbrain	Present	Regionally restricted	3I	Expression was detected in dorsal and ventral longitudinal domains in the superior colliculus.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	3J	Expression was detected in the commissural pretectum.
TS21: diencephalon	Strong	Regionally restricted	3J	Expression was strong in basal prosomeres 1-3.
TS21: hypothalamus	Present	Regionally restricted	3J	Expression was detected in the anterior hypothalamus.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Strong	Regionally restricted	3K	Expression was strong in basal prosomeres 2-3.
TS21: midbrain	Present	Regionally restricted	3K	Expression was detected in dorsal and ventral longitudinal domains in the superior colliculus and in the basal plate of the midbrain tegmentum.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata alar plate	Present		4C
TS21: medulla oblongata basal plate	Present		4C

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		4D
TS21: medulla oblongata alar plate	Present		4D
TS21: medulla oblongata basal plate	Present		4D

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata alar plate	Present		4E
TS21: medulla oblongata basal plate	Present		4E

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		4F
TS21: medulla oblongata alar plate	Present		4F
TS21: medulla oblongata basal plate	Present		4F

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	6B	Expression was detected in the commissural pretectum and in the basal plate of the midbrain tegmentum.