Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85738
MacLean HE, et al., Absence of transcription factor c-maf causes abnormal terminal differentiation of hypertrophic chondrocytes during endochondral bone development. Dev Biol. 2003 Oct 1;262(1):51-63
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5505429
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Probe:
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2ar cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
1D E14.5:
embryonic day 14.5
(more  )
Specimen
1D E14.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
1D E15.5:
embryonic day 15.5
(more )
Specimen
1D E15.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Present |
Regionally restricted |
1D E15.5
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Expression was detected in only terminally differentiated hypertrophic chondrocytes.
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Specimen
1D E16.5:
embryonic day 16.5
(more )
Specimen
1D E16.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
1D E16.5
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Expression was detected in only terminally differentiated hypertrophic chondrocytes. Expression was also in the osteoblasts in the primary spongiosa.
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Specimen
1D E17.5:
embryonic day 17.5
(more )
Specimen
1D E17.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Present |
Regionally restricted |
1D E17.5
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Expression was detected in only terminally differentiated hypertrophic chondrocytes. Expression was also in the osteoblasts in the primary spongiosa.
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Specimen
1D E18.5:
embryonic day 18.5
(more )
Specimen
1D E18.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
1D E18.5
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Expression was detected in only terminally differentiated hypertrophic chondrocytes. Expression was also in the osteoblasts in the primary spongiosa.
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Specimen
1D 24d:
postnatal day 24
(more )
Specimen
1D 24d:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 24
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
1D 24d
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Expression was detected in only terminally differentiated hypertrophic chondrocytes. Expression was also in the osteoblasts in the primary spongiosa.
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Specimen
3B +/+:
embryonic day 15.5
(more )
Specimen
3B +/+:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Present |
Regionally restricted |
3B +/+ osteopontin
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Expression was detected in terminal hypertrophic chondrocytes.
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Specimen
3B -/-:
(close )
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Genetic Background:
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involves: BALB/c
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Mutant Allele(s):
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Maftm1Glm/Maftm1Glm
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tibia |
Present |
Regionally restricted |
3B -/- osteopontin
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Expression intensity in terminal hypertrophic chondrocytes was normal, but the domain was decreased in the mutant.
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Specimen
3C +/+:
embryonic day 16.5
(more )
Specimen
3C +/+:
(close )
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Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
3C +/+ osteopontin
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Expression was detected in terminal hypertrophic chondrocytes.
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Specimen
3C -/-:
(close )
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Genetic Background:
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involves: BALB/c
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Mutant Allele(s):
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Maftm1Glm/Maftm1Glm
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tibia |
Present |
Regionally restricted |
3C -/- osteopontin
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The expression domain in terminal hypertrophic chondrocytes expanded in the mutant at E16.5.
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Analysis Tools