Gene Expression Data
Assay Details
Assay
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Reference:
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J:85738
MacLean HE, et al., Absence of transcription factor c-maf causes abnormal terminal differentiation of hypertrophic chondrocytes during endochondral bone development. Dev Biol. 2003 Oct 1;262(1):51-63
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5505428
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Gene symbol:
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Col10a1
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Gene name:
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collagen, type X, alpha 1
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Probe:
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Col10a1 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
1C E14.5:
embryonic day 14.5
(more )
Specimen
1C E14.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: tibia cartilage condensation |
Present |
Regionally restricted |
1C E14.5
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Expression was detected by all hypertrophic chondrocytes, at high levels in early hypertrophic cells and lower levels in late hypertrophic chondrocytes.
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Specimen
1C E15.5:
embryonic day 15.5
(more )
Specimen
1C E15.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: tibia |
Present |
Regionally restricted |
1C E15.5
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Expression was detected by all hypertrophic chondrocytes, at high levels in early hypertrophic cells and lower levels in late hypertrophic chondrocytes.
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Specimen
1C E16.5:
embryonic day 16.5
(more )
Specimen
1C E16.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: tibia |
Present |
Regionally restricted |
1C E16.5
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Expression was detected by all hypertrophic chondrocytes, at high levels in early hypertrophic cells and lower levels in late hypertrophic chondrocytes.
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Specimen
1C E17.5:
embryonic day 17.5
(more )
Specimen
1C E17.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: tibia |
Present |
Regionally restricted |
1C E17.5
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Expression was detected by all hypertrophic chondrocytes, at high levels in early hypertrophic cells and lower levels in late hypertrophic chondrocytes.
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Specimen
1C E18.5:
embryonic day 18.5
(more )
Specimen
1C E18.5:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: tibia |
Present |
Regionally restricted |
1C E18.5
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Expression was detected by all hypertrophic chondrocytes, at high levels in early hypertrophic cells and lower levels in late hypertrophic chondrocytes.
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Specimen
1C 24d:
postnatal day 24
(more )
Specimen
1C 24d:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 24
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: tibia |
Present |
Regionally restricted |
1C 24d
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Expression was detected by all hypertrophic chondrocytes, at high levels in early hypertrophic cells and lower levels in late hypertrophic chondrocytes.
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Specimen
3A +/+:
embryonic day 14.5
(more )
Specimen
3A +/+:
(close )
Genetic Background:
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involves: BALB/c
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
3A -/-:
(close )
Genetic Background:
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involves: BALB/c
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Mutant Allele(s):
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Maftm1Glm/Maftm1Glm
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
3B +/+:
embryonic day 15.5
(more )
Specimen
3B +/+:
(close )
Genetic Background:
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involves: BALB/c
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Age:
|
embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: tibia |
Present |
Regionally restricted |
3B +/+ collagen X
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Expression was detected in hypertrophic chondrocytes.
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Specimen
3B -/-:
(close )
Genetic Background:
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involves: BALB/c
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Mutant Allele(s):
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Maftm1Glm/Maftm1Glm
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: tibia |
Present |
Regionally restricted |
3B -/- collagen X
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Expression intensity in hypertrophic chondrocytes was normal, but the domain was decreased in the mutant.
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Specimen
3C +/+:
embryonic day 16.5
(more )
Specimen
3C +/+:
(close )
Genetic Background:
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involves: BALB/c
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: tibia |
Present |
Regionally restricted |
3C +/+ collagen X
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Expression was detected in hypertrophic chondrocytes.
|
Specimen
3C -/-:
(close )
Genetic Background:
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involves: BALB/c
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Mutant Allele(s):
|
Maftm1Glm/Maftm1Glm
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: tibia |
Present |
Regionally restricted |
3C -/- collagen X
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The expression domain in hypertrophic chondrocytes expanded in the mutant at E16.5.
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