Probe:	Robo1 probe2
Probe preparation:	labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Absent		3M
TS15: dermomyotome	Present	Regionally restricted	3M	Expression in entire dermomyotome except within the intersomite boundary.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3N	Expression in spinal cord in motor columns and commissural neurons.
TS17: neural tube floor plate	Absent		3N
TS17: neural tube roof plate	Absent		3N
TS17: dermomyotome	Present	Regionally restricted	3N	Expression in entire dermomyotome except within the intersomite boundary.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	3O	Expression in spinal cord in motor columns and commissural neurons.
TS19: neural tube basal columns	Present		3O
TS19: neural tube floor plate	Absent		3O
TS19: neural tube roof plate	Absent		3O

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	3P	Expression in spinal cord in motor columns and commissural neurons.
TS21: basal columns	Present		3P
TS21: spinal cord floor plate	Absent		3P
TS21: spinal cord roof plate	Absent		3P

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	4M	Expression in midbrain ventricular zone.
TS13: inner ear	Present	Regionally restricted	4M	Expression in otic vesicle.
TS13: 1st branchial arch	Present	Regionally restricted	4M	Expression in distal tip of first branchial a

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	4N	Expression in midbrain ventricular zone.
TS14: inner ear	Present	Regionally restricted	4N	Expression in otic vesicle.
TS14: 1st branchial arch	Present	Regionally restricted	4N	Expression in distal tip of first branchial arch.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	4O	Expression in midbrain ventricular zone
TS15: 1st branchial arch	Strong		4O	Prominent expression in first branchial arch.
TS15: otocyst	Present	Regionally restricted	4O	Expression forming a ring outlining the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Strong	Regionally restricted	4P	Prominent expression in ventricular zone of telencephalon.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Strong		5K
TS17: 1st branchial arch maxillary component	Strong		5K
TS17: nasal process	Strong	Regionally restricted	5K	Expression in groove between the lateral nasal process.
TS17: limb	Strong	Regionally restricted	5K	Prominent expression in proximal mesenchyme of limb bud.
TS17: midbrain	Weak		5K
TS17: head	Weak	Regionally restricted	5K	Weak expression in neural groove between nasal process and telencephalon.
TS17: dermomyotome	Present	Regionally restricted	5K	Expression in entire dermomyotome except within the intersomite boundary.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	5L	Expression in both mandibular and maxillary components of first branchial arch.
TS19: branchial arch mesenchyme	Strong		5L
TS19: telencephalon	Present		5L
TS19: latero-nasal process	Present	Regionally restricted	5L	Expression in groove between the lateral nasal process.
TS19: limb mesenchyme	Present	Regionally restricted	5L	Expression in proximal mesenchyme of limb bud.
TS19: midbrain	Weak		5L
TS19: head	Weak	Regionally restricted	5L	Weak expression in neural groove between nasal process and telencephalon.
TS19: sclerotome	Present	Regionally restricted	5L	Expression in segmented pattern in anterior sclerotome.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	6C	Expression in proximal mesenchyme of limb bud.

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	6F	Expression at tip of digits.

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	6F inset	Expression at tip of digits.

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Present	Regionally restricted	6I	Expression in segmented pattern in anterior sclerotome.

Structure	Level	Pattern	Image	Note
TS19: dermomyotome	Present	Regionally restricted	6L	Expression in entire dermomyotome except within the intersomite boundary.
TS19: sclerotome	Present	Regionally restricted	6L	Expression in medial and lateral sclerotome.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	7D	Expression in inner nuclear layer of retina.
TS23: optic nerve	Weak		7D

Structure	Level	Pattern	Image	Note
TS23: vibrissa follicle	Present	Regionally restricted	7G	Expression in whisker follicle in the bulb. Weak expression in dermal papilla and hair root.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present		8D
TS23: olfactory epithelium	Present		8D
TS23: ganglionic eminence	Present	Regionally restricted	8D	Expression in intermediate zone of ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS25: cochlea	Present		8H
TS25: lens	Present		8H
TS25: neural retina	Present		8H
TS25: olfactory epithelium	Present		8H
TS25: trigeminal ganglion	Present		8H