Probe:	Sox10 probe8
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: rhombomere neural crest	Present		2J +/+

Structure	Level	Pattern	Image	Note
TS12: future midbrain neural crest	Present		6A +/+ 4s	Expression found in nascent neural crest cells as they migrate from the midbrain.
TS12: migrating neural crest	Present		6A +/+ 4s	Expression found in nascent neural crest cells as they migrate from the midbrain.

Structure	Level	Pattern	Image	Note
TS12: future midbrain neural crest	Present		6A -/- 5s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.
TS12: migrating neural crest	Present		6A -/- 5s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.

Structure	Level	Pattern	Image	Note
TS13: future midbrain neural crest	Present		6A +/+ 10s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS13: future prosencephalon neural crest	Present		6A +/+ 10s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS13: future hindbrain neural crest	Present	Regionally restricted	6A +/+ 10s	Expression found in migratory neural crest cells originating from the upper hindbrain.
TS13: rhombomere neural crest	Present	Regionally restricted	6A +/+ 10s	Expression found in migratory neural crest cells originating from the postotic rhombomeres.
TS13: migrating neural crest	Present	Regionally restricted	6A +/+ 10s	There were 2 distinct streams of neural crest cells that remained separate from one another, leaving a discrete Sox10-free zone lateral to rhomobomere 3.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural crest	Present		6A -/- 8s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.
TS13: future midbrain neural crest	Present		6A -/- 8s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.
TS13: future prosencephalon neural crest	Present		6A -/- 8s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.
TS13: migrating neural crest	Present		6A -/- 8s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.
TS13: rhombomere neural crest	Present		6A -/- 8s	Expressing cells emerged on time and at the correct sites from the neural plate in a similar manner to the neural crest cells of the wild type.

Structure	Level	Pattern	Image	Note
TS13: future midbrain neural crest	Present		6A +/+ 12s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS13: future prosencephalon neural crest	Present		6A +/+ 12s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS13: future hindbrain neural crest	Present	Regionally restricted	6A +/+ 12s	Expression found in migratory neural crest cells originating from the upper hindbrain.
TS13: rhombomere neural crest	Present	Regionally restricted	6A +/+ 12s	Expression found in migratory neural crest cells originating from the postotic rhombomeres.
TS13: facial neural crest	Weak	Regionally restricted	6A +/+ 12s	Expression was down-regulated in neural crest cells in the frontonasal and maxillary region.
TS13: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6A +/+ 12s	Expression was detected in neural crest cells streaming from the lower midbrain and rhombomeres 1-2 to the first branchial arch.
TS13: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6A +/+ 12s	Expression was detected in neural crest cells streaming from rhombomeres 4-5 to the second branchial arch.
TS13: migrating neural crest	Present	Regionally restricted	6A +/+ 12s	There were 2 distinct streams of neural crest cells that remained separate from one another, leaving a discrete Sox10-free zone lateral to rhomobomere 3.

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch mesenchyme derived from neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: 2nd branchial arch mesenchyme derived from neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: facial neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: future hindbrain neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: future midbrain neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: future prosencephalon neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: migrating neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS13: rhombomere neural crest	Present		6A -/- 11s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.

Structure	Level	Pattern	Image	Note
TS14: future midbrain neural crest	Present		6A +/+ 14s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS14: prosencephalon neural crest	Present		6A +/+ 14s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS14: future hindbrain neural crest	Present	Regionally restricted	6A +/+ 14s	Expression found in migratory neural crest cells originating from the upper hindbrain.
TS14: rhombomere	Present	Regionally restricted	6A +/+ 14s	Expression found in migratory neural crest cells originating from the postotic rhombomeres.
TS14: facial neural crest	Weak	Regionally restricted	6A +/+ 14s	Expression was down-regulated in neural crest cells in the frontonasal and maxillary region.
TS14: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6A +/+ 14s	Expression was detected in neural crest cells streaming from the lower midbrain and rhombomeres 1-2 to the first branchial arch.
TS14: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6A +/+ 14s	Expression was detected in neural crest cells streaming from rhombomeres 4-5 to the second branchial arch.
TS14: migrating neural crest	Present	Regionally restricted	6A +/+ 14s	There were 2 distinct streams of neural crest cells that remained separate from one another, leaving a discrete Sox10-free zone lateral to rhomobomere 3.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mesenchyme derived from neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: 2nd branchial arch mesenchyme derived from neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: facial neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: future hindbrain neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: future midbrain neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: migrating neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: prosencephalon neural crest	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.
TS14: rhombomere	Present		6A -/- 14s	Expressing cells emerged on time and at the correct sites from the neural plate and were distributed to the facial tissue and branchial arches in a similar manner to the neural crest cells of the wild type.

Structure	Level	Pattern	Image	Note
TS14: future midbrain neural crest	Present		6A +/+ 17s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS14: prosencephalon neural crest	Present		6A +/+ 17s	Expression found in migratory neural crest cells originating from the forebrain and midbrain.
TS14: future hindbrain neural crest	Present	Regionally restricted	6A +/+ 17s	Expression found in migratory neural crest cells originating from the upper hindbrain.
TS14: rhombomere	Present	Regionally restricted	6A +/+ 17s	Expression found in migratory neural crest cells originating from the postotic rhombomeres.
TS14: facial neural crest	Weak	Regionally restricted	6A +/+ 17s	Expression was down-regulated in neural crest cells in the frontonasal and maxillary region.
TS14: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6A +/+ 17s	Expression was detected in neural crest cells streaming from the lower midbrain and rhombomeres 1-2 to the first branchial arch.
TS14: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6A +/+ 17s	Expression was detected in neural crest cells streaming from rhombomeres 4-5 to the second branchial arch.
TS14: migrating neural crest	Present	Regionally restricted	6A +/+ 17s	There were 2 distinct streams of neural crest cells that remained separate from one another, leaving a discrete Sox10-free zone lateral to rhomobomere 3.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mesenchyme derived from neural crest	Present		6A -/- 17s
TS14: 2nd branchial arch mesenchyme derived from neural crest	Present		6A -/- 17s
TS14: migrating neural crest	Present		6A -/- 17s	Late-migrating populations of neural crest cells merged as they streamed toward the branchial arches. The Sox10-free zone is lost.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	6A +/+ 20s	There were 2 distinct streams of neural crest cells that remained separate from one another, leaving a discrete Sox10-free zone lateral to rhomobomere 3.
TS14: branchial arch mesenchyme derived from neural crest	Weak	Regionally restricted	6A +/+ 20s	Expression was progressively down-regulated in the postmigratory neural crest cells that populate the branchial arches.

Structure	Level	Pattern	Image	Note
TS14: migrating neural crest	Present	Regionally restricted	6A -/- 20s	Late-migrating populations of neural crest cells merged as they streamed toward the branchial arches. The Sox10-free zone is lost.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	6A +/+ 22s	There were 2 distinct streams of neural crest cells that remained separate from one another, leaving a discrete Sox10-free zone lateral to rhomobomere 3.
TS15: branchial arch mesenchyme derived from neural crest	Weak	Regionally restricted	6A +/+ 22s	Expression was progressively down-regulated in the postmigratory neural crest cells that populate the branchial arches.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	6A -/- 22s	Late-migrating populations of neural crest cells merged as they streamed toward the branchial arches. The Sox10-free zone is lost. Neural crest cells in the periocular region didn't downregulate expression.
TS15: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present	Regionally restricted	6A -/- 22s	Neural crest cells in the maxillary and mandibular primordia didn't downregulate expression.
TS15: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present	Regionally restricted	6A -/- 22s	Neural crest cells in the maxillary and mandibular primordia didn't downregulate expression.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present	Regionally restricted	6A +/+ 27s	Expression was detected in the progenitors of the cranial nerve ganglion.
TS15: branchial arch mesenchyme derived from neural crest	Weak	Regionally restricted	6A +/+ 27s	Expression was progressively down-regulated in the postmigratory neural crest cells that populate the branchial arches.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	6A -/- 27s	Late-migrating populations of neural crest cells merged as they streamed toward the branchial arches. The Sox10-free zone is lost. Neural crest cells in the periocular region didn't downregulate expression.
TS15: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present	Regionally restricted	6A -/- 27s	Neural crest cells in the maxillary and mandibular primordia didn't downregulate expression.
TS15: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present	Regionally restricted	6A -/- 27s	Neural crest cells in the maxillary and mandibular primordia didn't downregulate expression.

Structure	Level	Pattern	Image	Note
TS16: cranial ganglion	Present	Regionally restricted	6A +/+ 32s	Expression was detected in the progenitors of the cranial nerve ganglion.

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present	Regionally restricted	6A -/- 30s	Late-migrating populations of neural crest cells merged as they streamed toward the branchial arches. The Sox10-free zone is lost. Neural crest cells in the periocular region didn't downregulate expression.
TS16: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present	Regionally restricted	6A -/- 30s	Neural crest cells in the maxillary and mandibular primordia didn't downregulate expression.
TS16: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present	Regionally restricted	6A -/- 30s	Neural crest cells in the maxillary and mandibular primordia didn't downregulate expression.

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme	Present	Regionally restricted	6B	Expression was detected in the subectodermal site in the cranial mesenchyme.

Structure	Level	Pattern	Image	Note
TS14: head paraxial mesenchyme	Present	Regionally restricted	6C	Expression showed the intrusion of neural crest cells into the paraxial mesenchyme in the mutant embryo.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component	Present	Non-Uniform	6D	Expressing and non-expressing cells intermingled throughout the arch with a slight tendency to localize in the lateral subectodermal region.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component	Weak	Regionally restricted	6E	Expression was weaker, and the cells were distributed more to the lateral region of the mandibular process in the mutant.