Probe:	pW11.6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: truncus arteriosus	Present	Regionally restricted	4A	Expressed in the truncus arteriosus of the primitive heart tube.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present	Regionally restricted	4B	Expressed in the truncus arteriosus.
TS15: somite	Present	Graded	4B	Expression is strongest in the first somite pair and progressively weaker in each successive somite.
TS15: forelimb bud	Present		4B

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	4C	Expressed in the dorsal surface of the limb buds, and along the anterior side of the bud.
TS17: hindlimb bud	Present	Regionally restricted	4C	Expressed in the dorsal surface of the limb buds, and along the anterior side of the bud.

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present		4D	Expressed in the mesenchymal regions of both the developing limb stalk and the foot paddle.
TS19: future arm mesenchyme	Present		4D	Expressed in the mesenchymal regions of both the developing limb stalk and the foot paddle.
TS19: future leg mesenchyme	Present		4D	Expressed in the mesenchymal regions of both the developing limb stalk and the foot paddle.
TS19: handplate mesenchyme	Present		4D	Expressed in the mesenchymal regions of both the developing limb stalk and the foot paddle.
TS19: maxillary process	Present	Regionally restricted	4D	Expressed in the maxillary component of the first branchial arch.
TS19: latero-nasal process	Present	Regionally restricted	4D	Expressed in the lateral nasal process on either side of the nasolacrymal groove.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Regionally restricted	4E	Expression in the distal limb is restricted to the rostral and caudal margins of each developing digits.
TS20: hindlimb digit mesenchyme	Present	Regionally restricted	4E	Expression in the distal limb is restricted to the rostral and caudal margins of each developing digits.
TS20: perioptic mesenchyme	Present	Regionally restricted	4E	Expressed in regions surrounding the eye.

Structure	Level	Pattern	Image	Note
TS21: foot pre-cartilage condensation	Present	Regionally restricted	4F	Expressed in the developing perichondrium of each forming digit.
TS21: hand pre-cartilage condensation	Present	Regionally restricted	4F	Expressed in the developing perichondrium of each forming digit.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	5A	Expression is initially restricted to the crania-medial corner of the somite. As the somite matures, expression expands to cover the entire length of the medial edge of the somite at the junction of the dermatome and myotome.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		5B	Expressed in the myocardial layer of the heart wall.

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: outflow tract	Present	Regionally restricted		Expressed in the truncus arteriosus of the primitive heart tube.
TS13: somite	Present	Regionally restricted		Expressed along the medial edge of the several most rostral somites.