Probe:	Raldh2 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Bright-field view for histology (left side) and the corresponding dark-field view (right side).

Structure	Level	Pattern	Image	Note
TS12: extraembryonic mesenchyme	Absent		3A
TS12: node	Absent		3A

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Strong	Regionally restricted	3B	Expression is along the dorsal edges of the coelomic cavity.
TS17: trunk	Strong	Regionally restricted	3B	Expression is around the mesonephric ducts, close to the mesonephric ducts entry site into the cloaca.
TS17: mesonephros mesenchyme	Present		3B
TS17: stomach	Present	Regionally restricted	3B
TS17: midgut mesenchyme	Present	Regionally restricted	3B	Expression is adjacent to the stomach.
TS17: mesonephric tubule	Absent		3B
TS17: nephric duct	Absent		3B

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Strong	Regionally restricted	3C	Expression is along the dorsal edges of the coelomic cavity.
TS17: mesonephros mesenchyme	Present		3C
TS17: forelimb bud	Absent		3C
TS17: hindlimb bud	Absent		3C
TS17: mesonephric tubule	Absent		3C

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	3D	Expression is in the lateral quadrant.

Structure	Level	Pattern	Image	Note
TS20: intraembryonic coelom	Present		3E
TS20: mesonephros	Present		3E
TS20: midgut	Present		3E
TS20: trunk	Strong	Regionally restricted	3E	Expression is around the cloaca, extending up to the base of the genital tubercle.
TS20: genital tubercle	Strong	Regionally restricted	3E	Expression is at the base.

Structure	Level	Pattern	Image	Note
TS15: foregut	Present	Regionally restricted		Expression is in the dorsal region of the primitive stomach mesenchyme.
TS15: nervous system	Absent
TS15: optic vesicle	Absent
TS15: otocyst	Absent

Structure	Level	Pattern	Image	Note
TS19: nose	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS21: intraembryonic coelom	Present
TS21: midgut	Present
TS21: oral region	Present	Regionally restricted		Expression is near the lateral clefts of the mouth cavity.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	4A	Expression is in a proximal domain in a zone between the limb buds and flank region and in a central domain around the main arteries of the buds.
TS19: footplate mesenchyme	Present	Regionally restricted	4A	Expresion is in a distal domain in the presumptive interdigital regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	4B	Expression is in cervical and lumbar regions adjacent to the developing limbs in clusters in the ventral horns.
TS20: forelimb mesenchyme	Present	Regionally restricted	4B	Expression is in a central domain around the developing limb arterial system.
TS20: pericardium	Present		4B
TS20: pleura	Present		4B
TS20: forelimb interdigital region	Strong		4B

Structure	Level	Pattern	Image	Note
TS25: forelimb digit	Present	Regionally restricted	4C	Expression is in the dorsal perichondrial cells of the terminal phalanges.

Structure	Level	Pattern	Image	Note
TS19: maxillary artery	Strong		6A
TS19: maxillary process	Strong		6A
TS19: trigeminal ganglion	Strong		6A

Structure	Level	Pattern	Image	Note
TS20: oral region	Present	Regionally restricted	6B	Expression is below the cleft separating the tongue from the mandibular region.
TS20: head mesenchyme	Present	Regionally restricted	6B	Expression is in the loose, vascular mesenchyme surrounding the eyes.

Structure	Level	Pattern	Image	Note
TS21: esophagus mesenchyme	Strong		6C
TS21: larynx aditus	Present	Regionally restricted	6C	Expression is in the pharyngeal-laryngeal region at the base of the tongue.
TS21: pharynx	Present	Regionally restricted	6C	Expression is in the pharyngeal-laryngeal region at the base of the tongue.
TS21: nasal cavity	Present	Regionally restricted	6C	Expression is in the mesenchyme surrounding the olfactory epithelium.
TS21: meninges	Present		6C
TS21: pituitary gland	Present		6C

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Present	Regionally restricted	6D	Expression is in the loose, vascular mesenchyme surrounding the eyes.
TS22: eye	Present	Regionally restricted	6D	Expression is in retrolenticular mesenchyme.
TS22: perioptic mesenchyme	Present		6D
TS22: lens	Absent		6D
TS22: neural retinal epithelium	Absent		6D

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Strong	Regionally restricted	6E
TS26: adenohypophysis	Present		6E
TS26: meninges	Present		6E
TS26: neurohypophysis	Weak		6E

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present		6F
TS26: dental sac	Present		6F

Structure	Level	Pattern	Image	Note
TS21: metanephros	Strong		7A
TS21: pericardio-peritoneal canal mesothelium	Present	Regionally restricted	7A
TS21: pleura	Present	Regionally restricted	7A
TS21: urogenital sinus	Present		7A
TS21: intestine mesenchyme	Present		7A	Expression is in the mesenchyme of the intestinal loops.
TS21: heart	Absent		7A
TS21: liver	Absent		7A
TS21: lung	Absent		7A

Structure	Level	Pattern	Image	Note
TS22: esophagus mesenchyme	Strong		7B
TS22: pericardio-peritoneal canal mesothelium	Present		7B
TS22: pleura	Present		7B
TS22: renal cortex	Present		7B
TS22: stomach	Present	Regionally restricted	7B

Structure	Level	Pattern	Image	Note
TS26: esophagus mesenchyme	Present		7C
TS26: intestine mesenchyme	Present		7C
TS26: seminiferous cord	Present		7C
TS26: stomach mesenchyme	Present		7C
TS26: bladder	Present	Regionally restricted	7C	Expression is in cells underlying the urinary bladder epithelium.
TS26: reproductive system	Strong	Regionally restricted	7C	Expression is around the lower genital tract.
TS26: epididymis	Strong		7C