Probe:	c-kit probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS25: choroid plexus	Present		1A
TS25: cortical plate	Present		1A
TS25: hippocampus	Present		1A
TS25: hypothalamus	Present		1A
TS25: liver	Present		1A
TS25: main olfactory bulb	Present		1A
TS25: hindbrain	Present	Regionally restricted	1A	Expression in the rhombencephalic lip.

Structure	Level	Pattern	Image	Note
TS24: skin	Absent		1C
TS24: liver	Weak		1C	Reduced expression relative to that observed in wild type or heterozygous littermates.
TS24: gut	Present		1C
TS24: lung	Present		1C
TS24: nasal cavity epithelium	Present		1C

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Strong		2B left
TS21: hindbrain ventricular layer	Strong		2B left	Authors report high levels of expression in the ventricular zone of the fourth ventricle.
TS21: brain ventricular layer	Present	Regionally restricted	2B left	Lower levels of expression in ventricular zones of the other ventricles.
TS21: dorsal root ganglion	Present		2B left
TS21: liver	Present		2B left

Structure	Level	Pattern	Image	Note
TS21: brain ventricular layer	Present		2B right	Expression in the brain indistinguishable from those of normal embryos.
TS21: liver	Weak		2B right	Markedly reduced expression relative to that in wild type or heterozygous littermates.
TS21: cranial ganglion	Present	Regionally restricted	2B right	Expression in neural-crest derived cranial ganglia
TS21: dorsal root ganglion	Present		2B right

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	3A
TS28: germ cell of ovary	Present	Regionally restricted	3A	Authors report expression in germ cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Present		3D

Structure	Level	Pattern	Image	Note
TS28: interstitium of the testis	Present		3G left

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Present		3G right	Expression in Leydig cells not significantly different relative to control embryos.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		4A/E

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	4C/G maternal	High levels of expression in mesometrial decidual cells.

Structure	Level	Pattern	Image	Note
TS14: chorioallantoic placenta	Present	Regionally restricted	4C/G extraembryonic	Authors report expression in the chorion.
TS14: ectoplacental cone	Present		4C/G extraembryonic

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		4I maternal	High expression in maternal decidual cells.

Structure	Level	Pattern	Image	Note
TS21: spongiotrophoblast	Weak		4I extraembryonic

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		4K maternal	Expression indistinguishable from those of normal mice.

Structure	Level	Pattern	Image	Note
TS21: extraembryonic component	Present		4K extraembryonic	Expression indistinguishable from those of normal mice.

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present	Regionally restricted		Expression in neural-crest derived cranial ganglia.
TS20: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS23: cranial ganglion	Present	Regionally restricted		Expression in neural-crest derived cranial ganglia.
TS23: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS24: cranial ganglion	Present	Regionally restricted	5A	Expression in neural-crest derived cranial ganglia.
TS24: dorsal root ganglion	Present		5A
TS24: skin	Absent		5A	No expression in any tissue normally colonized by neural crest-derived malanoblasts.

Structure	Level	Pattern	Image	Note
TS24: cranial ganglion	Present	Regionally restricted	5C	Expression in neural-crest derived cranial ganglia.
TS24: dorsal root ganglion	Present		5C
TS24: skin	Absent		5C	No expression in any tissue normally colonized by neural crest-derived malanoblasts.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		6A

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted	7A	Expression located close to junction between external plexiform and glomerular layers, and superficial half of the external plexiform layer.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present	Punctate	7D	Expression in punctate cells distributed mainly in layers 1 to 3.
TS28: cortical layer II	Present	Punctate	7D	Expression in punctate cells distributed mainly in layers 1 to 3.
TS28: cortical layer III	Present	Punctate	7D	Expression in punctate cells distributed mainly in layers 1 to 3.
TS28: cortical layer IV	Weak		7D	Lesser extent of expression in layers 4 to 6.
TS28: cortical layer V	Weak		7D	Lesser extent of expression in layers 4 to 6.
TS28: cortical layer VI	Weak		7D	Lesser extent of expression in layers 4 to 6.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Scattered	8B	Expression in dispersed glial cells in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present		8D	Authors state they were unable to observe any changes in expression relative to normal mice.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present		8F	Authors state they were unable to observe any changes in expression relative to normal mice.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		8H
TS28: hippocampus CA3	Present	Regionally restricted	8H	Expression in CA3 pyramidal neurons.
TS28: subiculum	Present	Regionally restricted	8H

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		8J	Authors state they were unable to observe any changes in expression relative to normal mice.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		8L	Authors state they were unable to observe any changes in expression relative to normal mice.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted		Expression probably in superficial short axon neurons, possibly some glial cells.
TS28: neocortex layer	Strong	Scattered		Expression in scattered cells in layers 1-6; glial cells at least in layer 1.
TS28: corpus striatum	Weak	Scattered		Expression in a few scattered neurons.
TS28: telencephalon	Strong	Scattered		Expression in scattered cells in the paleocortex.
TS28: hippocampus pyramidal cell layer	Strong	Scattered		Expression in pyramidal neurons of hippocampus layers CA1 and CA3
TS28: hippocampus CA2	Weak
TS28: presubiculum	Present
TS28: subiculum	Present
TS28: hippocampal formation	Present	Regionally restricted		Expression in the parasubiculum.
TS28: thalamus	Absent
TS28: hypothalamus	Present
TS28: telencephalon septum	Absent
TS28: inferior colliculus	Present
TS28: superior colliculus	Present	Regionally restricted		Expression in the rostral superior colliculi.
TS28: red nucleus	Weak
TS28: deep cerebellar nucleus	Present	Regionally restricted