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Gene Expression Data
Assay Details
Assay
Reference: J:164222 Beverdam A, et al., Protein tyrosine kinase 2 beta (PTK2B), but not focal adhesion kinase (FAK), is expressed in a sexually dimorphic pattern in developing mouse gonads. Dev Dyn. 2010 Oct;239(10):2735-41
Assay type: RT-PCR
MGI Accession ID: MGI:5302108
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Probe: Sox9-pG, Sox9-pH
Probe preparation: labelled with SYBR green
Assay notes: This is a quantitative RT-PCR. 18S ribosomal RNA was used as the standard. QRT-PCR primers were designed to generate 60-145 bp amplicons spanning introns.
Results
Image: 3_Sox9 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
XY +/+ E12.5 TS20: testis Present (a) Not Specified; total RNA CD-1 Male
XY -/- E12.5 TS20: testis Not Specified (b) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 Sox9tm1Gsr/Sox9tm1Gsr, Krt19tm1(cre)Mmt/Krt19+Male Conditional mutant.
XX +/+ E12.5 TS20: ovary Weak Not Specified; total RNA CD-1 Female
Notes:
(a) Relatively high levels compared to female.
(b) Severely reduced expression relative to male wild-type. Lower than those in female wild-type.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory