Probe:	Leprel2Sma probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Weak		5a
TS15: hindlimb ridge	Weak		5a
TS15: 1st branchial arch	Present		5a
TS15: branchial arch	Weak		5a	There is weak expression in anterior craniofacial/branchial arch tissue.
TS15: head	Weak		5a	There is weak expression in anterior craniofacial/branchial arch tissue.
TS15: embryo ectoderm	Weak		5a	There is weak expression in ectodermal tissue.
TS15: otocyst	Absent		5a	There is no expression in the otic capsule.

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		5b
TS15: paraxial mesenchyme	Present		5b
TS15: branchial arch	Weak		5b	There is weak expression in anterior craniofacial/branchial arch tissue.
TS15: head	Weak		5b	There is weak expression in anterior craniofacial/branchial arch tissue.
TS15: embryo ectoderm	Weak		5b	There is weak expression in ectodermal tissue.
TS15: otocyst	Absent		5b	There is no expression in the otic capsule.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Weak		5d
TS17: hindlimb bud	Weak		5d
TS17: 1st branchial arch	Present		5d
TS17: paraxial mesenchyme	Present		5d
TS17: 1st branchial arch maxillary component	Weak		5d
TS17: fronto-nasal process	Weak		5d

Structure	Level	Pattern	Image	Note
TS17: notochord	Present		5e , 5f	Expression is present in the anterior notochord.

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Present		5g

Structure	Level	Pattern	Image	Note
TS17: paraxial mesenchyme	Present		5h

Structure	Level	Pattern	Image	Note
TS19: embryo	Present		5j	Expression is markedly reduced across all axial levels compared to earlier gestational times.
TS19: forelimb bud	Present		5j
TS19: hindlimb bud	Present		5j
TS19: branchial arch	Present		5j
TS19: fronto-nasal process	Present		5j	Expression is present in frontal nasal tissues.
TS19: paraxial mesenchyme	Present		5j	Expression is waning along the rostral-caudal axis.

Structure	Level	Pattern	Image	Note
TS19: notochord	Absent		5l , 5k	There is no expression in the anterior domains.

Structure	Level	Pattern	Image	Note
TS19: notochord	Trace	Regionally restricted	5m , 5n	There is minimal expression in caudal domains at the level of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS17: notochord	Present		6a' Leprecan-like2 E10.5	Inset shows higher magnification of staining in the notochord.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		6a' Leprecan-like2 E11.5
TS19: dorsal root ganglion	Present		6a' Leprecan-like2 E11.5
TS19: neural tube floor plate	Present		6a' Leprecan-like2 E11.5

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		6a' Leprecan-like2 E12.5
TS20: dorsal root ganglion	Present		6a' Leprecan-like2 E12.5
TS20: spinal cord floor plate	Present		6a' Leprecan-like2 E12.5

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted	6a' Leprecan-like2 E13.5	Expression is present in the notochord.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		6b'n	Expression is present in the cartilage of the proximal forelimb.

Structure	Level	Pattern	Image	Note
TS20: mandibular process mesenchyme	Present		6b'o

Structure	Level	Pattern	Image	Note
TS20: eye	Present		6b'p

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		6b'q

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present		6b'r

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	6b's	Expression is present in the neural arch cartilages.
TS21: dorsal root ganglion	Present		6b's

Structure	Level	Pattern	Image	Note
TS21: renal medulla	Present	Regionally restricted	6b't	Expression is present in epithelial cells lining the future ureteric ducts. Shown at higher magnification in the red inset.
TS21: mesenchyme of metanephros	Present		6b't	Expression is present in the nephrogenic mesenchyme of the developing kidney. Shown at higher magnification in the blue inset.