Probe:	Vangl2 cDNA3
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: nervous system	Very strong	Widespread	1G	Expression was very strong in all neural structures, particularly in ventricular zones.
TS17: limb	Moderate		1G
TS17: tail	Moderate		1G

Structure	Level	Pattern	Image	Note
TS20: nervous system	Very strong		2Q
TS20: skin	Moderate		2Q
TS20: embryo mesenchyme	Moderate	Regionally restricted	2Q	Expression was moderate in subcutaneous mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Very strong		2R
TS20: skin	Moderate		2R
TS20: embryo mesenchyme	Moderate	Regionally restricted	2R	Expression was moderate in subcutaneous mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		2S , 2T	Expression was evenly distributed, somewhat higher in ventricular zone and postmitotic differentiation fields.
TS20: brain ventricular layer	Present		2S , 2T	Expression was evenly distributed, somewhat higher in ventricular zone and postmitotic differentiation fields.

Structure	Level	Pattern	Image	Note
TS22: nervous system	Strong		3F
TS22: brain ventricular layer	Present	Graded	3F	Expression was detected in the ventricular zone in all sectors of the brain, with a caudal low to rostral high gradient.

Structure	Level	Pattern	Image	Note
TS25: brainstem	Ambiguous	Regionally restricted		Expression was present in the cochleovestibular nuclei and very low or undetectable in the reticular formation.
TS25: cerebellum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the external granule layer.
TS25: midbrain ventricular layer	Strong
TS25: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS25: midbrain tegmentum	Ambiguous			Expression was very low or undetectable.
TS25: tectum	Ambiguous			Expression was very low or undetectable.
TS25: diencephalon ventricular layer	Present
TS25: thalamus	Ambiguous	Regionally restricted		Expression was very low or undetectable in dorsal and ventral thalamus.
TS25: retina	Strong	Regionally restricted		Expression was high in the ventricular zone and ganglion cells.
TS25: cortical plate	Present	Regionally restricted		Expression was high in the cortical plate ventricular zone/subventricular zone and present in the cortical plate proper.
TS25: corpus striatum	Present	Regionally restricted		Expression was high in the striatum ventricular zone/subventricular zone and present in the striatum.
TS25: main olfactory bulb	Present	Regionally restricted		Expression was detected in the olfactory bulb and mitral cells.

Structure	Level	Pattern	Image	Note
TS25: brain	Present	Regionally restricted	4F2	Expression was more intense in ventricular zone than in differentiating neuronal fields.
TS25: brain ventricular layer	Present		4F2	Expression was more intense in ventricular zone than in differentiating neuronal fields.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Moderate	Regionally restricted	4F3	Expression was detected in the medial and dorsal sectors of the cortical plate.
TS25: brain	Present	Regionally restricted	4F3	Expression was more intense in ventricular zone than in differentiating neuronal fields.
TS25: brain ventricular layer	Present		4F3	Expression was more intense in ventricular zone than in differentiating neuronal fields.

Structure	Level	Pattern	Image	Note
TS25: olfactory epithelium	Strong		4F4
TS25: retina	Strong		4F4
TS25: skin	Strong		4F4
TS25: tooth	Strong		4F4

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Regionally restricted	5J	Expression was robust in periventricular zones and moderate in neuronal maturation fields.
TS27: cerebral cortex	Present	Regionally restricted	5J	Expression was robust in periventricular zones and moderate in neuronal maturation fields.
TS27: hippocampus	Present	Regionally restricted	5J	Expression was robust in periventricular zones and moderate in neuronal maturation fields.
TS27: rostral migratory stream	Present		5J
TS27: cerebellum external granule cell layer	Present		5J

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong
TS28: diencephalon	Present	Regionally restricted		Expression was detected in the diencephalon ventricular zone.
TS28: dentate gyrus	Present
TS28: dorsal thalamus	Present
TS28: hippocampus pyramidal cell layer	Present
TS28: hypothalamus	Present
TS28: prethalamus	Present
TS28: cerebral cortex	Present	Regionally restricted		Expression was detected in the cortical plate proper but was very low or undetectable in the cortical plate ventricular zone/subventricular zone.
TS28: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum and the ventricular zone/subventricular zone.
TS28: rostral migratory stream	Present	Regionally restricted
TS28: main olfactory bulb granule cell layer	Ambiguous			Expression was very low or undetectable.
TS28: main olfactory bulb mitral cell layer	Ambiguous			Expression was very low or undetectable.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		6P
TS28: brain	Present	Regionally restricted	6P	Expression was detected in the periventricular layer.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		6Q

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present		6R

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7F
TS28: brain	Present	Regionally restricted	7F	Expression was diffuse with significant labelling in the residual ventricular zone around the lateral ventricles.