Col5a3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:61325 Imamura Y, et al., The pro-alpha3(V) collagen chain. Complete primary structure, expression domains in adult and developing tissues, and comparison to the structures and expression domains of the other types V and XI procollagen chains. J Biol Chem. 2000 Mar 24;275(12):8749-59
Assay type:	RNA in situ
MGI Accession ID:	MGI:4848142
Gene symbol:	Col5a3
Gene name:	collagen, type V, alpha 3

Probe:	Col5a3 cDNA3
Probe preparation:	Antisense labelled with S35 RNA

Results

Specimen not shown: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: embryo	Absent

Specimen not shown: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: embryo	Trace

Specimen 5 pro-alpha3 (V) top: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: embryo mesenchyme	Present	Regionally restricted	5_pro-alpha3(V) top	Expression was in superficial fascia and epimysia of developing muscles of the anterior chest wall, in presumptive ligamentous attachments apposed to cartilage primordia of the exoccipital bone, basioccipital bone, vertebrae C1 and C2, and hip joint.
TS23: hindlimb mesenchyme	Present	Regionally restricted	5_pro-alpha3(V) top	Expression was detected within tendons of the hindlimb.

Specimen 5 pro-alpha3 (V) bottom: embryonic day 15.5 (more

)
Note: Higher magnification images were shown in Fig. 6.

Structure	Level	Pattern	Image	Note
TS23: embryo mesenchyme	Present	Regionally restricted	6_pro-alpha3 (V) top left , 5_pro-alpha3(V) bottom , 6_pro-alpha3 (V) top right , 6_pro-alpha3 (V) bottom	Expression was in superficial fascia and epimysia of developing muscles of the anterior chest wall, in presumptive ligamentous attachments apposed to cartilage primordia of the hip joint, and in the connective tissue sheath, or epineureum, of some nerves.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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